This column has been replaced by
the new FlexFire Series.

Develosil UHPLC Series

Introducing a new UHPLC Series dedicated for UHPLC analysis.

Complete solution using only one type of particle.

Designed for UHPLC analysis, the Develosil UHPLC Series uses pure 1.6 µm silica particles. C30, C18, C8, C1 and HILIC functional groups provide the ability to tune selectivity and retention time for a wide range of analytes.

Working on hydrophilic compounds? Start with C18, and then try C8 or C1 based on the required retention. When targeting high polar compounds, C30 or C1 will show more characteristic separation. To retain highly polar compounds that normally elute near the front peak, choose HILIC and a highly organic initial mobile phase.

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Develosil UHPLC Series Specifications

Product	UHPLC C30	UHPLC C18	UHPLC C8	UHPLC C1	UHPLC HILIC
Functional Group	Triacontyl	Octadecyl	Octyl	Trimethyl	–
Particle Size	1.6 µm
Surface Area	340 m²/g
Pore Volume	1.0 mL/g
Pore Diameter	11 nm
Carbon	13%	17%	12%	5.5%	–
Endcapping	Yes				–
pH Range	pH 1-9				pH 2-7
Temperature Range	Max 60°C
Maximum Pressure	80 Mpa

Applications

Analysis of Peptides with UHPLC

UHPLC Oligonucleotide Analysis

Ultra-Pure Silica Gel Material

The Develosil UHPLC Series utilizes our purest silica yet. This selection further reduces unnecessary metal coordination and results in sharper peak shapes. Moreover, this selection results in high tolerance to the extreme pressure ranges encountered in UHPLC.

Analytical Conditions
Column	Develosil UHPLC HILIC, 3 µm (4.6 x 150 mm) Develosil Silica 100, 3 µm ( 4.6 x 150 mm)
Mobile Phase	Acetronitrile/water = 90/10
Flow Rate	1.0 mL/min Detection CAD
Temperature	40°C
Sample	Allantoin (0.5 mg/mL)
Injection Volume	1.0 µL

Establish New Mobile Phase Conditions
with More Variety in pH.

The Develosil UHPLC Series opens the way to a dynamic pH change of your mobile phase conditions. C30 provides more dynamic change on its retention comparing to C18. It is possible to achieve the optimal retention and separation for highly polar compounds by changing the pH of your mobile phase. This feature will help you create favorable LC/MS conditions in a short time.

Changing the pH can easily change the pattern of a chromatogram’s profile. In addition to this, establishing a new analytical method that does not require ion pairing reagents can greatly improve the column’s loading capacity and cleaning efficacy.

Analytical Conditions
Column	Develosil UHPLC C30 1.6 µm (2.0 x 50 mm)
Mobile Phase	Acetronitrile/ 10 mM HCOONH4 pH7.0=60/40 Acetonitrile/10 mM HCOONH4, pH3.0=60/40
low Rate	0.5 mL/min
Detection	UV254 nm
Temperature	40°C
Sample	Uracil (24 µg/mL) Prapranolol (82 µg/mL) Amitriptyline (68 µg/mL)
Injection Volume	0.08 µL