Develosil UHPLC Series

Develosil C30 Product Brochure

Introducing a new UHPLC Series dedicated for UHPLC analysis.

Complete solution using only one type of particle.

Designed for UHPLC analysis, the Develosil UHPLC Series uses pure 1.6 µm silica particles. C30, C18, C8, C1 and HILIC functional groups provide the ability to tune selectivity and retention time for a wide range of analytes.

Working on hydrophilic compounds? Start with C18, and then try C8 or C1 based on the required retention. When targeting high polar compounds, C30 or C1 will show more characteristic separation. To retain highly polar compounds that normally elute near the front peak, choose HILIC and a highly organic initial mobile phase.

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Develosil UHPLC Series Specifications

ProductUHPLC C30UHPLC C18UHPLC C8UHPLC C1UHPLC HILIC
Functional GroupTriacontylOctadecylOctylTrimethyl–
Particle Size1.6 µm
Surface Area340 m2/g
Pore Volume1.0 mL/g
Pore Diameter11 nm
Carbon13%17%12%5.5%–
EndcappingYes–
pH RangepH 1-9pH 2-7
Temperature RangeMax 60°C
Maximum Pressure80 Mpa

 

Applications

 

Analysis of Peptides with UHPLC

UHPLC Oligonucleotide Analysis

Ultra-Pure Silica Gel Material

The Develosil UHPLC Series utilizes our purest silica yet. This selection further reduces unnecessary metal coordination and results in sharper peak shapes. Moreover, this selection results in high tolerance to the extreme pressure ranges encountered in UHPLC.

Analytical Conditions
Column
  • Develosil UHPLC
    HILIC, 3 µm
    (4.6 x 150 mm)
  • Develosil Silica 100,
    3 µm ( 4.6 x 150 mm)
Mobile PhaseAcetronitrile/water = 90/10
Flow Rate1.0 mL/min
Detection CAD
Temperature40°C
SampleAllantoin (0.5 mg/mL)
Injection Volume1.0 µL

 

Establish New Mobile Phase Conditions
with More Variety in pH.

The Develosil UHPLC Series opens the way to a dynamic pH change of your mobile phase conditions. C30 provides more dynamic change on its retention comparing to C18. It is possible to achieve the optimal retention and separation for highly polar compounds by changing the pH of your mobile phase. This feature will help you create favorable LC/MS conditions in a short time.

Changing the pH can easily change the pattern of a chromatogram’s profile. In addition to this, establishing a new analytical method that does not require ion pairing reagents can greatly improve the column’s loading capacity and cleaning efficacy.

Analytical Conditions
ColumnDevelosil UHPLC C30 1.6 µm (2.0 x 50 mm)
Mobile Phase
  • Acetronitrile/ 10 mM HCOONH4 pH7.0=60/40
  • Acetonitrile/10 mM HCOONH4, pH3.0=60/40
low Rate0.5 mL/min
DetectionUV254 nm
Temperature40°C
Sample
  1. Uracil (24 µg/mL)
  2. Prapranolol (82 µg/mL)
  3. Amitriptyline (68 µg/mL)
Injection Volume0.08 µL

 

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Download the product brochure or browse our technical library to find additional applications and publications.