HSR C18 

Develosil HSR C18 Product Brochure
The Develosil HSR C18 is your go-to C18 column. It supports all HPLC analyses with high selectivity and high separation performance. This ODS column uses our own-production Si gel that has a large surface area to give you overwhelming retention capacity for your HPLC separations. The Develosil HSR C18 is subjected to a special end cap treatment that offers good peak shape in acidic, basic, or neutral conditions.

Develosil HSR C18 Specifications

Ligand Carbon End Capping Surface Area Pore Diameter Pore Volume Range of pH Column Withstand Pressure
ODS/C18 (Polymeric) 22% Yes 450 m²/g 80 Å 1.15 ml/g pH 1-10 25 MPa

Featured Applications

Separation Comparison of Pesticides

Separation Comparison of Pesticides using HPLC-UV
HPLC Analytical Conditions:
Column:  Develosil HSR C18, 5 µm (4.6 x 150 mm)
Mobile Phase: Acetonitrile/25mM NH₄H₂PO₄, pH=2.0=45/55
Flow Rate: 1.0 mL/min
Detection: UV at 210 nm
Sample: 1. Asulam (0.98 mg/mL)
2. Thiuram (1.12 mg/mL)
3. Triclopyr (1.01 mg/mL)
4. Flazasulfuron (0.97 mg/mL)
5. MCPP (0.97 mg/mL)
6. Halosulfuron-methyl (1.06 mg/mL)
Injection Volume: 2.0 µL

Capsaicin in Food

Analysis of Capsaicin in Food using HPLC-UV
HPLC Analytical Conditions:
Column:  Develosil HSR C18, 5 µm (4.6 x 150 mm)
Mobile Phase: Acetonitrile/0.1% Phosphoric Acid=60/40
Flow Rate: 1.0 mL/min
Detection: UV at 280 nm
Sample: 1. Capsaicin (0.59 mg/mL)
2. Dihydrocapsaicin (0.55 mg/mL)
Injection Volume: Food sample: 10 µL
Standard: 2.0 µL

Tricyclic Antidepressants

Analysis of Tricyclic Antidepressants using HPLC-UV

HPLC Analytical Conditions:

Column:  Develosil HSR C18, 5 µm (4.6 x 150 mm)
Mobile Phase: (MeOH ver.)
MeOH/25mM (NH₄)₂HPO₄, pH7.0=80/20
(Acetonitrile ver.)
ACN/25mM (NH₄)₂HPO₄, pH7.0=60/40
Flow Rate: 1.0 mL/min
Detection: UV at 280 nm
Sample: 1.Desipramine HCl (64ug/mL)
2.Nortriptyline HCl (52ug/mL)
3.Imipramine HCl (54ug/mL)
4.Amitriptyline HCl (53ug/mL)
Injection Volume:  2.0 µL

Download the product brochure or browse our technical library to find additional applications and publications.