HSR C18 

Develosil HSR C18 Product Brochure
The Develosil HSR C18 is your go-to C18 column. It supports all HPLC analyses with high selectivity and high separation performance. This ODS column uses our own-production Si gel that has a large surface area to give you overwhelming retention capacity for your HPLC separations. The Develosil HSR C18 is subjected to a special end cap treatment that offers good peak shape in acidic, basic, or neutral conditions.

Develosil HSR C18 Specifications

LigandCarbonEnd CappingSurface AreaPore DiameterPore VolumeRange of pHColumn Withstand Pressure
ODS/C18 (Polymeric)22%Yes450 m²/g80 Å1.15 ml/gpH 1-1025 MPa

Featured Applications

Separation Comparison of Pesticides

Separation Comparison of Pesticides using HPLC-UV
HPLC Analytical Conditions:
Column: Develosil HSR C18, 5 µm (4.6 x 150 mm)
Mobile Phase:Acetonitrile/25mM NH₄H₂PO₄, pH=2.0=45/55
Flow Rate:1.0 mL/min
Detection:UV at 210 nm
Sample:1. Asulam (0.98 mg/mL)
2. Thiuram (1.12 mg/mL)
3. Triclopyr (1.01 mg/mL)
4. Flazasulfuron (0.97 mg/mL)
5. MCPP (0.97 mg/mL)
6. Halosulfuron-methyl (1.06 mg/mL)
Injection Volume:2.0 µL

Capsaicin in Food

Analysis of Capsaicin in Food using HPLC-UV
HPLC Analytical Conditions:
Column: Develosil HSR C18, 5 µm (4.6 x 150 mm)
Mobile Phase:Acetonitrile/0.1% Phosphoric Acid=60/40
Flow Rate:1.0 mL/min
Detection:UV at 280 nm
Sample:1. Capsaicin (0.59 mg/mL)
2. Dihydrocapsaicin (0.55 mg/mL)
Injection Volume:Food sample: 10 µL
Standard: 2.0 µL

Tricyclic Antidepressants

Analysis of Tricyclic Antidepressants using HPLC-UV

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm (4.6 x 150 mm)
Mobile Phase:(MeOH ver.)
MeOH/25mM (NH₄)₂HPO₄, pH7.0=80/20
(Acetonitrile ver.)
ACN/25mM (NH₄)₂HPO₄, pH7.0=60/40
Flow Rate:1.0 mL/min
Detection:UV at 280 nm
Sample:1.Desipramine HCl (64ug/mL)
2.Nortriptyline HCl (52ug/mL)
3.Imipramine HCl (54ug/mL)
4.Amitriptyline HCl (53ug/mL)
Injection Volume: 2.0 µL

Download the product brochure or browse our technical library to find additional applications and publications.