HSR C18 Peptide - Develosil USA

HSR C18 Peptide

Develosoil HSR C18 Peptide Product Brochure

The Develosil HSR C18 Peptide was specifically designed for your analysis of amino acids, peptides, and nucleic acids. This column adopts the HSR series’s refined silica gel base material and is Develosil’s first column where functional group density is controlled. The Develosil HSR C18’s newly constructed bonding technology greatly contributes to durability and smoother method development.


**Metal-Free option available!

Develosil HSR C18 Peptide Specifications

Ligand Carbon End Capping Surface Area Pore Diameter pH range Temperature Range Particle Size
Silica (ODS)
17% Yes 300 m²/g 14 nm pH 1-10 5°-80°C 2.5 µm , 3 µm, 5 µm

Featured Applications

Separation Comparison of Peptides

Separation Comparison of Peptides using HPLC-UV
HPLC Analytical Conditions:
Column: Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)

Develosil HSR C18 Peptide, 5 µm (4.6 x 150 mm)

Other company 300 Å C18, 5 µm (4.6 x 150 mm)

Mobile Phase: A) Water 0.1% TFA
B) Acetonitrile +0.1% TFA
Gradient: B) 0%-50%(0-30 min), 50%-0%(30.1-40 min), 0%(40.0min-)
Flow Rate: 1.0 mL/min
Temperature: 50°C
Detection: UV at 210 nm
Sample: 1. Gly-Tyr
2. Val-Tyr-Val
3. Met-Enkephalin
4. Angiotensin II
5. Leu-Enkephalin
6.  γ-Endorphin
7. ß-Endorphin
8. Insultin
Injection Volume: 20.0 µL
System Thermofisher SCIENTIFIC Ultimate 3000
Mixer Volume 150 µL

Separation Comparison of dNTP

Separation Comparison of dNTP using HPLC-UV
HPLC Analytical Conditions:
Column:  C18 Column, 5 µm (4.6 x 150 mm)
Mobile Phase: 25mM Phosphate Buffer. pH=7.0
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 254 nm
Sample: 1. dCTP (0.13 mM)
2. dTTP (0.13 mM)
3. dGTP (0.13 mM)
4. dATP (0.13 mM)
Injection Volume: 2.0 µL

Download the product brochure or browse our technical library to find additional applications and publications.