HSR AQ C18, HSR C1 

Develosil HSR AQ C18, C1 Product Brochure

The Develosil AQ HSR C18 and Develosil HSR C1 help you separate high polarity compounds on a reverse phase column. The Develosil HSR AQ C18 and Develosil HSR C1 gives you maximum performance even in 100% aqueous mobile phase conditions.

As being part of the HSR series, these columns are subjected to original end cap technology to maximize peak shape in acidic or basic conditions.

Develosil HSR AQ C18 and HSR C1 Specifications

 

Column Ligand Carbon End Capping Surface Area Pore Diameter Range of pH Particle Size
HSR AQ C18 ODS/C18 18% Yes 450 m2/g 100 Å pH 2-9 2 µm, 3 µm, 5 µm
HSR C1 TMS/C1 6.5% pH 2-8 3 µm, 5 µm

Featured Applications

Analysis of Water Soluble Vitamins

Analysis of Water Soluble Vitamins using HPLC-UV
HPLC Analytical Conditions:
Column:  Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
Mobile Phase: A) 25mM NH₄H₂PO₄ (pH=3.0)
B)  Methanol
Gradient: B) 5%(0-2min), 5%-50%(2-7min), 50%(7-10min), 5%(10.1min-)​
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 210 nm
Sample: 1. Thiamin HCl (0.48mg/mL)
2. L(+)-Ascorbic acid (0.49mg/mL)
3. Nicotinic acid (0.49mg/mL)
4. Pyridoxine HCl (0.46mg/mL)
5. Nicotinamide (0.50mg/mL)
6. Calcium (+)-pantothenate (0.65mg/mL)
7. Cyanocobalamin (0.46mg/mL)
8. Folic acid (0.46mg/mL)
9. Riboflavin sodium phosphate (0.46mg/mL)
10. (+)-Biotin (0.47mg/mL)
Injection Volume: 1.0 µL

Analysis of Catechin in Green Tea

Analysis of Catechin in Green Tea using HPLC-UV
HPLC Analytical Conditions:
Column:  1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)

2. Develosil HSR C1, 5 µm (4.6 x 150 mm)

Mobile Phase: Methanol/25mM NH₄H₂PO₄ (pH=2.0)=25/75
Gradient: B) 5%(0-2min), 5%-50%(2-7min), 50%(7-10min), 5%(10.1min-)​
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 280 nm
Sample: 1. (-)-Epigallocatechin (0.25mg/mL)
2. (-)-Epigallocatechin gallate (0.30mg/mL)
3. Caffeine
4. (-)-Epicatechin (0.25mg/mL)
5. (-)-Epicatechin gallate (0.30mg/mL)
Injection Volume: 2.0 µL

Analysis of Peptides

Analysis of Peptides using HPLC

HPLC Analytical Conditions:

Column:  1. Develosil HSR C1, 5 µm (4.6 x 150 mm)
2. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
Mobile Phase: A. 25 mM NaCl (pH 3.0)
B) Acetonitrile
Flow Rate: 1.0 mL/min
Gradient: See more data here.
Temperature 30°C
Sample: 1.Gly-Tyr (0.05mg/mL)
2.Val-Tyr-Val (0.05mg/mL)
3.Met-Enkephalin (0.05mg/mL)
4.Angiotensin II (0.05mg/mL)
5.Leu-Enkephalin (0.05mg/mL)
Injection Volume:  10.0 µL

Download the product brochure or browse our technical library to find additional applications and publications.