Diol Series
The Develosil 100 Diol and 300 Diol was designed for gel filtration using
Other typical uses for this column is size exclusion chromatography of large water-soluble compounds such as proteins and enzymes.
For low molecular compounds, we recommend the 100 Diol; and for large macromolecular compounds, we recommend the 300 Diol.
Develosil Diol Series Specifications
| Column Name | Ligand | Carbon | End Capping | Surface Area | Pore Diameter | Pore Volume | Range of pH |
|---|---|---|---|---|---|---|---|
| 100 Diol | Glycero propyl radical | 12% | No | 350 m²/g | 120 Å | 1.05 ml/g | pH 2-7.5 |
| 300 Diol | 9% | 180 m²/g | 250 Å |
Size Exclusion Limit for Develosil Diol Series
| Column Name | Proteinic Exclusion Limit Molecualr Weight | Polyethylene Glycol Exclusion Limit Molecular Weight |
|---|---|---|
| 100 Diol | 100,000 | 10,000 |
| 300 Diol | 1,000,000 | 100,000 |
Feautured Users
Identification of Flavo-3-ols in Avocado using HPLC-FLD
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil 100 Diol 5 µm (250 x 4.6 mm) |
| Mobile Phase: | A. Acidic Acetonitrile (ACN:Acetic Acid, 98:2, v/v) B. Acidic aqueous methanol (MeOH:Water:Acetic Acid, 95:3:2, v/v) |
| Gradient: | 1. 7% B for 3 min 2. 37.6% B for 57 min. 3. 100% B for 7 min. 4. 7% B for 5 min. |
| Detection: | Fluorescence Detector (Excitation wavelength-230 nm; Emission wavelength 321 nm) |
| Sample: | Various Degrees of Polymerization of Flavo-3-ols in: 1. Avocado Pulp 2. Avocado Skin 3. Avocado Seed |
| Injection Volume: | 5.0 µL |
Ana López-Cobo, Ana María Gómez-Caravaca, Federica Pasini, María Fiorenza Caboni, Antonio Segura-Carretero, Alberto Fernández-Gutiérrez, HPLC-DAD-ESI-QTOF-MS and HPLC-FLD-MS as valuable tools for the determination of phenolic and other polar compounds in the edible part and by-products of avocado, LWT, Volume 73, 2016, Pages 505-513, ISSN 0023-6438, https://doi.org/10.1016/j.lwt.2016.06.049.
Analysis of Procyanidins in Cocoa using HILIC-FLD
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil 100 Diol, 5 µm (250 mm x 1mm) |
| Mobile Phase: | A. Acidic Acetonitrile (ACN B. Acidic aqueous methanol (MeOH:Water:Acetic Acid, 94.05:4.95:1, v/v) |
| Detection: | Fluorescence Detector (Excitation wavelength-230 nm; Emission wavelength 321 nm) |
| Sample: | Various Degrees of Polymerization of |
| Other analytical conditions can be found in | |
Kathithileni M. Kalili, André de Villiers, Systematic optimisation and evaluation of on-line, off-line and stop-flow comprehensive hydrophilic interaction chromatography×reversed phase liquid chromatographic analysis of procyanidins, Part I: Theoretical considerations,
Journal of Chromatography A, Volume 1289, 2013, Pages 58-68, ISSN 0021-9673, https://doi.org/10.1016/j.chroma.2013.03.008.
Download the product brochure or browse our technical library to find additional applications and publications.