Diol Series

Develosil Diol Series Product Brochure

The Develosil 100 Diol and 300 Diol was designed for gel filtration using Develosil’s high quality Si gel. This column is used worldwide in food research specifically with proanthocyanidins.

Other typical uses for this column is size exclusion chromatography of large water-soluble compounds such as proteins and enzymes.

For low molecular compounds, we recommend the 100 Diol; and for large macromolecular, we recommend the 300 Diol.

Develosil Diol Series Specifications

Column Name Ligand Carbon End Capping Surface Area Pore Diameter Pore Volume Range of pH
100 Diol Glycero propyl
radical
12% No 350 m²/g 120 Å 1.05 ml/g pH 2-7.5
300 Diol 9% 180 m²/g 250 Å

Size Exclusion Limit for Develosil Diol Series

Column Name Proteinic Exclusion Limit Molecualr Weight Polyethylene Glycol Exclusion Limit Molecular Weight
100 Diol 100,000 10,000
300 Diol 1,000,000 100,000

Feautured Users

Identification of Flavo-3-ols in Avocado using HPLC-FLD

Identification of Flavo-3-ols in Avocado using HPLC-FLD
HPLC Analytical Conditions:
Column:  Develosil 100 Diol 5 µm (250 x 4.6 mm)
Mobile Phase: A. Acidic Acetonitrile (ACN:Acetic Acid, 98:2, v/v)
B. Acidic aqueous methanol (MeOH:Water:Acetic Acid, 95:3:2, v/v)
Gradient: 1. 7% B for 3 min
2. 37.6% B for 57 min.
3. 100% B for 7 min.
4. 7% B for 5 min.
Detection: Fluorescence Detector
(Excitation wavelength-230 nm;
Emission wavelength 321 nm)
Sample: Various Degrees of Polymerization of Flavo-3-ols in:
1. Avocado Pulp
2. Avocado Skin
3. Avocado Seed
Injection Volume: 5.0 µL

Data from:

Ana López-Cobo, Ana María Gómez-Caravaca, Federica Pasini, María Fiorenza Caboni, Antonio Segura-Carretero, Alberto Fernández-Gutiérrez, HPLC-DAD-ESI-QTOF-MS and HPLC-FLD-MS as valuable tools for the determination of phenolic and other polar compounds in the edible part and by-products of avocado, LWT, Volume 73, 2016, Pages 505-513, ISSN 0023-6438, https://doi.org/10.1016/j.lwt.2016.06.049.

Analysis of Procyanidins in Cocoa using HILIC-FLD

Analysis of Procyandins in Cocoa using HILIC-FLD
HPLC Analytical Conditions:
Column:  Develosil 100 DIol, 5 µm (250 mm x 1mm)
Mobile Phase: A. Acidic Acetonitrile (ACN:Acetic Acid, 99:1, v/v)
B. Acidic aqueous methanol (MeOH:Water:Acetic Acid, 94.05:4.95:1, v/v)
Detection: Fluorescence Detector
(Excitation wavelength-230 nm;
Emission wavelength 321 nm)
Sample: Various Degrees of Polymerization of procyandin oligomers in cocoa extract
Other analytical conditions can be found in article.

Data from:

Kathithileni M. Kalili, André de Villiers, Systematic optimisation and evaluation of on-line, off-line and stop-flow comprehensive hydrophilic interaction chromatography×reversed phase liquid chromatographic analysis of procyanidins, Part I: Theoretical considerations,
Journal of Chromatography A, Volume 1289, 2013, Pages 58-68, ISSN 0021-9673, https://doi.org/10.1016/j.chroma.2013.03.008.

 

Download the product brochure or browse our technical library to find additional applications and publications.