Diol Series
The Develosil 100 Diol and 300 Diol was designed for gel filtration using Develosil’s high quality Si gel. This column is used worldwide in food research specifically with proanthocyanidins.
Other typical uses for this column is size exclusion chromatography of large water-soluble compounds such as proteins and enzymes.
For low molecular compounds, we recommend the 100 Diol; and for large macromolecular, we recommend the 300 Diol.
Develosil Diol Series Specifications
| Column Name | Ligand | Carbon | End Capping | Surface Area | Pore Diameter | Pore Volume | Range of pH |
|---|---|---|---|---|---|---|---|
| 100 Diol | Glycero propyl radical |
12% | No | 350 m²/g | 120 Å | 1.05 ml/g | pH 2-7.5 |
| 300 Diol | 9% | 180 m²/g | 250 Å |
Size Exclusion Limit for Develosil Diol Series
| Column Name | Proteinic Exclusion Limit Molecualr Weight | Polyethylene Glycol Exclusion Limit Molecular Weight |
|---|---|---|
| 100 Diol | 100,000 | 10,000 |
| 300 Diol | 1,000,000 | 100,000 |
Feautured Users
Identification of Flavo-3-ols in Avocado using HPLC-FLD
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil 100 Diol 5 µm (250 x 4.6 mm) |
| Mobile Phase: | A. Acidic Acetonitrile (ACN:Acetic Acid, 98:2, v/v) B. Acidic aqueous methanol (MeOH:Water:Acetic Acid, 95:3:2, v/v) |
| Gradient: | 1. 7% B for 3 min 2. 37.6% B for 57 min. 3. 100% B for 7 min. 4. 7% B for 5 min. |
| Detection: | Fluorescence Detector (Excitation wavelength-230 nm; Emission wavelength 321 nm) |
| Sample: | Various Degrees of Polymerization of Flavo-3-ols in: 1. Avocado Pulp 2. Avocado Skin 3. Avocado Seed |
| Injection Volume: | 5.0 µL |
Data from:
Ana López-Cobo, Ana María Gómez-Caravaca, Federica Pasini, María Fiorenza Caboni, Antonio Segura-Carretero, Alberto Fernández-Gutiérrez, HPLC-DAD-ESI-QTOF-MS and HPLC-FLD-MS as valuable tools for the determination of phenolic and other polar compounds in the edible part and by-products of avocado, LWT, Volume 73, 2016, Pages 505-513, ISSN 0023-6438, https://doi.org/10.1016/j.lwt.2016.06.049.
Analysis of Procyanidins in Cocoa using HILIC-FLD
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil 100 DIol, 5 µm (250 mm x 1mm) |
| Mobile Phase: | A. Acidic Acetonitrile (ACN:Acetic Acid, 99:1, v/v) B. Acidic aqueous methanol (MeOH:Water:Acetic Acid, 94.05:4.95:1, v/v) |
| Detection: | Fluorescence Detector (Excitation wavelength-230 nm; Emission wavelength 321 nm) |
| Sample: | Various Degrees of Polymerization of procyandin oligomers in cocoa extract |
| Other analytical conditions can be found in article. | |
Data from:
Kathithileni M. Kalili, André de Villiers, Systematic optimisation and evaluation of on-line, off-line and stop-flow comprehensive hydrophilic interaction chromatography×reversed phase liquid chromatographic analysis of procyanidins, Part I: Theoretical considerations,
Journal of Chromatography A, Volume 1289, 2013, Pages 58-68, ISSN 0021-9673, https://doi.org/10.1016/j.chroma.2013.03.008.
Download the product brochure or browse our technical library to find additional applications and publications.