C30 Series

Develosil C30 Product Brochure

Notably, Develosil is world famous for their C30 HPLC column!

Develosil C30 phases work with 100% aqueous mobile phase and gives added retention for highly polar compounds as well as provides great HPLC separations for liposolubility compounds like carotenoids.

Develosil C30 Series Specifications

Column Name Ligand Carbon End Capping Surface Area Pore Diameter Pore Volume Range of pH
C30-UG Triacontyl Radical
(Monofunctional)
18% Yes
(Double)
300 m2/g 14 nm 1.05 ml/g pH 2-8
RPAQUEOUS
RPFULLERENE
Combi-RP
RPAQUEOUS-AR Triacontyl Radical
(Trifunctional)
pH 1-8

Feautured Applications

Carotene Separation Comparison

Carotene Separation Comparison using HPLC-UV
HPLC Analytical Conditions:
Column:  1.Develosil RPAQUEOUS-AR-5 (4.6x250mm)
2. ODS (4.6x250mm)
Mobile Phase: Acetonitrile/ Chloroform=8/2
Flow Rate: 1.0 mL/min
Detection: UV at 450 nm
Sample: 1. α-carotene
2. ß-carotene

Nucleic Acid and Bases Separation Comparison

Nucleic Acid and Bases Separation Comparison using HPLC-UV
HPLC Analytical Conditions:
Column:  1. Develosil RPAQUEOUS-5 250 x 4.6 mm
2. Other C30 – 250 x 4.6 mm
Mobile Phase: 10 mM NaH2PO4 with H3PO4 (pH=7.0)
Flow Rate: 1.0 mL/min
Temperature 40°C
Detection: UV at 254 nm
Sample:
  1. Sodium Nitrite
  2. Cytosine
  3. Uracil
  4. Cytidin
  5. Uridine
  6. Thymine

Water Soluble Vitamins

Water Soluble Vitamins Analysis using HPLC-UV
HPLC Analytical Conditions:
Column:  Develosil RPAQUEOUS-5 150 x 4.6 mm
Mobile Phase: 50 mM sodium phosphate (pH=6.9)
Flow Rate: 1.0 mL/min
Detection: UV at 254 nm
Sample:
  1. Orotic acid
  2. Nicotinic acid
  3. Pyridoxal HCl
  4. Pyridoxine HCl
  5. Nicotinamide

Download the product brochure or browse our technical library to find additional applications and publications.