ANIDIUS

Product Brochure- Develosil ANIDIUS
The Develosil ANIDIUS was specifically designed for HILIC operations. The bonded phase is comprised of -OH and -NH2 groups and is bonded onto the silica base using a proprietary technology. The Develosil ANIDIUS is most suitable for high polarity compounds that cannot be separated using reverse phase chromatography.

Develosil ANIDIUS Specifications

Bonded Phase Carbon End Capping Surface Area Pore Diameter Pore Volume Range of pH Particle Size
-OH and -NH28 8% Yes 300 m²/g 140 Å 1.15 ml/g pH 2-7.5 5 µm

Featured Applications

Analysis of Saccharides 

Analysis of Saccharides using HPLC-RI Figure 1
HPLC Analytical Conditions:
Column:  Develosil ANIDIUS (4.6 x 250 mm)
Mobile Phase: Acetonitrile/Water=80/20
Flow Rate: 1.0 mL/min
Detection: RI
Sample: Fructose, Glucose, Sucrose, Maltose
Injection Volume: 20 µL
Analysis of Saccharides using HPLC-RI Figure 2
HPLC Analytical Conditions:
Column:  Develosil ANIDIUS (4.6 x 250 mm)
Mobile Phase: Acetonitrile/Water=65/35
Flow Rate: 1.0 mL/min
Detection: RI
Sample: Glucose, maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose
Injection Volume: 20 µL

Analysis of Water-soluble Vitamins

Analysis of Water Soluble Vitamins using HPLC-RI

HPLC Analytical Conditions:

Column:  Develosil ANIDIUS (4.6 x 250 mm)
Mobile Phase: A) Acetonitrile + 0.1% Formic Acid
B) 0.1% Formic Acid
Gradient: B) 25-60% (15 minutes) -> 60% (30mins)
Flow Rate: 0.5 mL/min
Detection: UV at 280 nm
Sample: 1. Thiamine (0.5 mg/mL)
2. Nicotineamide (1.0 mg/mL)
3. Riboflavin (1.0 mg/mL)
4. Cyancobalamin (1.0 mg/mL)
5. Nicotinic Acid (1.0 mg/mL)
6. Ascorbic acid (3.0 mg/mL)
Injection Volume:  2.0 µL

Download the product brochure or browse our technical library to find additional applications and publications.