FlexFire UHPLC and HPLC Columns | Develosil USA

UHPLC and HPLC Columns

The only brand to unite both types
of chromatography—seamlessly

  • Unmatched durability even for high pH applications
  • Unique transferability from R&D to QC

See for yourself:

In the world of research, you need to count on your UHPLC column to deliver precise, high-resolution, reproducible separations—especially if you work with large molecules, such as monocolonal antibodies, or use high-pH mobile phases.

In the world of production, you need your HPLC column to work just as hard. But why must your protocols differ from those upstream in R&D?

They don’t have to. Not anymore. Not with FlexFire.

FlexFire UHPLC and HPLC chromatography columns not only provide industry-leading resistance to highly-basic applications, but they’re the only brand which allows for similar protocols for UHPLC and HPLC, allowing for seamless transferability from R&D to QC, regardless of functional group or particle size.

You won’t find this combination of robustness and flexibility anywhere else on the market.

Superior silica at work

The FlexFire series of reverse-phase columns take advantage of Develosil’s premium silica: It’s the highest grade, uniform, high-density, low-metal silica chemistry on the market, as evidenced by its unparalleled performance and durability in high-pH applications.

  • Durable. Stable at pH 1 to pH 10
  • Flexible. Transfer from UHPLC to HPLC at 1.6, 2.6, and 5 µm, with functional groups from C1 to C30 and Hilic

We invite you to try out FlexFire, obligation-free, for 7 days. Click here to get started!


Who makes the strongest HPLC column?

See how FlexFire out-performs well-known competitors

  • 20% Stronger Columns
  • Smaller Particles, Greater Longevity
Column Types Particle Size (µm)
Develosil FlexFire 1.6
Brand A 1.7
Brand B 1.7
Brand C 1.8
Brand D 1.9
Column Functional Group C18
Size 2.0 x 50 mm
Mobile Phase Acetonitrile / 10 mM NH4OH, pH10.5=60/40
Flow Rate 0.5 mL/min
Temperature 40°C
Detection UV 254 nm
Sample Naphthalene (0.1 mg/mL)
Injection Volume 0.16 µL


Unprecedented transferability between UHPLC and HPLC

The FlexFire series provides 1.6, 2.6, and 5 µm particle sizes which work for your analytical systems in both UHPLC and HPLC. This gives you unprecedented power to employ the same line of chromatography columns in different departments, from R&D to QC.

Think of the advantages to your operation: FlexFire dramatically reduces the required time for tweaking the optimal condition from one particle size to another, thanks to the quality and homogeneity of its silica gel packing, end-capping, and bonding of functional groups. That’s the key to FlexFire’s predictable consistency across all columns.

Don’t believe it? Just look at the figures below, showing HETP and column pressure at different flow rates. FlexFire’s smaller particle size provides higher resolution across a wider range of flow rates.

Pressure and HETP vs Flow Rate for FlexFire C18

Column Develosil FlexFire C18 (2.0 x 50 mm)
Mobile Phase Acetonitrile/Water = 60/40
Temperature 40°C
Detection UV 254 nm
Sample Naphthalene
Injection Volume 0.16 µL

Different particle sizes, same resolution

Witness the flexibility to transfer between UHPLC and HPLC

FlexFire provides the same surface area ratio of 340 m2/g, along with the same physical properties, and the same bonding of functional group and end-capping—on three different particle-size silica substrates.

As a result, by choosing the correct column dimensions, you can maintain equivalent resolution, even when shifting between particle sizes. That’s the “Flex” in “FlexFire”: You can now easily determine the flow rate and gradient for different particle sizes with your chromatography software: No trial-and-error. No adjustments.

If you’re using UHPLC during R&D and HPLC during QC, FlexFire will save time previously spent re-adjusting the analytical conditions to find the best resolution. And if you’re planning to adopt UHPLC in the future, you can simply start with FlexFire 2.6 µm or 5 µm columns, knowing you’ll be “future-proofed” for UHPLC.

FlexFire C18, 5 µm (2.0 x 150 mm)

Gradient min mL/min %A %B Curve
0.00 0.189 90 10 6
8.44 0.189 75 25 6
16.88 0.189 75 25 6
17.06 0.189 90 10 6

Injection Volume: 1.2 µL

FlexFire C18, 2.6 µm (2.0 x 50 mm)

Gradient min mL/min %A %B Curve
0.00 0.364 90 10 6
1.46 0.364 75 25 6
2.93 0.364 75 25 6
2.96 0.364 90 10 6

Injection Volume: 0.4 µL

FlexFire C18, 1.6 µm (2.0 x 50 mm)

Gradient min mL/min %A %B Curve
0.00 0.592 90 10 6
0.90 0.592 75 25 6
1.80 0.592 75 25 6
1.82 0.592 90 10 6

Injection Volume: 0.4 µL
System:Waters ACQUITY UPLC H-Class Plus
Mixer: 100 µL

Mobile Phase A) 25mM HCOONH4, pH3.0
B) Acetonitrile
Temperature 40°C
Detection UV 260 nm
  1. Gly-Tyr
  2. Val-Tyr-Val
  3. Met-Enk
  4. Angiotensin II
  5. Leu-Enk

Specifications and Line Up

Function Particle Size Surface Area Pore Diameter Pore Volume Carbon Load pH Range Temp Tolerance
C18 1.6, 2.6,
and 5 µm
340 m2/g 11 nm 1.0 mL/g 22.0% 1-10 < 60°C
C30 11.00%
C8 12.00%
AQ C18 8.50%
C1 5.50%
C4 wide 2.6 and 5 µm 30 nm 1.4 mL/g 5.00% < 80°C
C4 mAb 100 nm 0.60%
120 SEC 5 µm 12 nm 1.0 mL/g 9.00% 2-10
300 SEC 5 µm 170 m2/g 30 nm 1.4 mL/g 6.00%

Pressure Tolerance

Particle Size For all Pore Sizes
1.6 µm 800 bar
2.6 µm 600 bar
5 µm 300 bar



Experience FlexFire For Yourself

You can’t appreciate FlexFire’s durability, robustness in high-pH environments, or unique ability to transfer between R&D and QC, without experiencing it firsthand.