Columns for UHPLC and HPLC

FlexFire, delivers supreme durability and maximum flexibility.

Develosil C30 Product Brochure

FlexFire’s value and performance lies in its high-quality silica, the lifeblood at the heart of its creation. Applying 40 years of manufacturing expertise and an upgraded production system, Develosil has upgraded to the highest grade uniform, high-density, low-metal porous silica. With improved endcapping technology Develosil withstands the harshest of conditions and delivers use after use, beyond the highest expectations.

With its availability in multiple functional groups and particle sizes, FlexFire is carefully designed for scalability and transferability to have your whole operation covered, from R&D to QC.

Find out more about FlexFire’s robust method development and transferability.

Durability

Column TypesParticle Size (µm)
Develosil FlexFire1.6
Brand A1.7
Brand B1.7
Brand C1.8
Brand D1.9
Conditions
Column Functional GroupC18
Size2.0 x 50 mm
Mobile PhaseAcetonitrile / 10 mM NH4OH, pH10.5=60/40
Flow Rate0.5 mL/min
Temperature40°C
DetectionUV 254 nm
SampleNaphthalene (0.1 mg/mL)
Injection Volume0.16 µL

Flexibility

HPLC-UHPLC Method Transferability

 FlexFire series provides 1.6, 2.6 and 5 µm particle sizes to fit the requirements of your analytical systems, both HPLC and UHPLC. This wide range of options allows the same line of columns to be used in different departments, such as R&D and QC (quality control).

 FlexFire dramatically shortens the required time for tweaking the optimal condition from one particle size to another. This is because the excellence and homogeneity of the silica gel, endcapping, and bonding of functional groups provides predictable consistency across all of the FlexFire columns.

 Figure below shows HETP and column pressure at different flow rates. Smaller particle size provides higher resolution across a wider range of flow rates.  

Pressure and HETP vs Flow Rate for FlexFire C18

Conditions
ColumnDevelosil FlexFire C18 (2.0 x 50 mm)
Mobile PhaseAcetonitrile/Water = 60/40
Temperature40°C
DetectionUV 254 nm
SampleNaphthalene
Injection Volume0.16 µL

Example of method transfer between HPLC and UHPLC

FlexFire displays the same surface area ratio of 340 m2/g, the same physical properties, and the same bonding of functional group and endcapping on three different particle size silica substrates.

As a result, by choosing the correct column dimensions users can maintain equivalent resolution while shifting between particle sizes. This great feature makes it simple to transfer methods between different particle sizes. Chromatography software can be used to determine the flow rate and gradient without trial and error, as no adjustments are necessary after the calculation.
If you are using UHPLC during R&D and HPLC during QC, FlexFire will save time previously spent re-adjusting the analytical conditions to find the best resolution. If you are planning to adopt UHPLC in the future, you can start with FlexFire 2.6 µm or 5 µm columns without worries.

FlexFire C18, 5 µm (2.0 x 150 mm)

GradientminmL/min%A%BCurve
0.000.18990106
8.440.18975256
16.880.18975256
17.060.18990106

Injection Volume: 1.2 µL

FlexFire C18, 2.6 µm (2.0 x 50 mm)

GradientminmL/min%A%BCurve
0.000.36490106
1.460.36475256
2.930.36475256
2.960.36490106

Injection Volume: 0.4 µL

FlexFire C18, 1.6 µm (2.0 x 50 mm)

GradientminmL/min%A%BCurve
0.000.59290106
0.900.59275256
1.800.59275256
1.820.59290106

Injection Volume: 0.4 µL
System:Waters ACQUITY UPLC H-Class Plus
Mixer: 100 µL

Conditions
Mobile PhaseA) 25mM HCOONH4, pH3.0
B) Acetonitrile
Temperature40°C
DetectionUV 260 nm
Sample
  1. Gly-Tyr
  2. Val-Tyr-Val
  3. Met-Enk
  4. Angiotensin II
  5. Leu-Enk

Specifications and Line Up

Function GroupParticle SizeSurface AreaPorous SizePorous VolumeCarbon LoadpH RangeWorking Temp
C181.6, 2.6, 5 µm340 m2/g11 nm1.0 mL/g22%pH1-10< 60°C
C3011%pH1-10
C8
C1
HILICpH1-5
Wide pore, low carbon C18, CN, PH, C4, NH2 columns will be introduced soon.

Pressure Tolerance

Particle SizeMax Pressure
1.6 µm11600 psi80 MPa800 bar
2.6 µm8700 psi60 MPa600 bar
5 µm4350 psi30 MPa300 bar