FlexFire Columns - Develosil USA

UHPLC and HPLC Columns

Achieving New Levels of

Freedom and Performance

Maintain performance while discovering your ideal conditions with Flexfire’s wide selection of HPLC and UHPLC columns.

Superior Silica

Bring high-density, and low-metal silica to your lab. FlexFire’s superior silica is evidenced by the ability to separate up to a 1-base difference in 20-base of nucleotides.
Column: FlexFire Column mAb-RP, 2.6 µm (2.0×50 mm)

For the best resolution and retention, we suggest using Diisopropanolamine (DIPA) as your ion-pair. For comparison, we have also displayed our results while using triethylamine (TEA).

Pore Size 1000 Å
Injection Volume 2.0 µL
Mixer 100 µL
Sample 20nt: 5′-GU ACG CGG AAU ACU UCG AdTdT-3′

21 nt: 5′-CGU ACG CGG AAU ACU UCG AdTdT-3′

Temperature 60°C
Detection UV260 nm
Mobile Phase TEA:

A) 100 mM HFIP + 10 mM TEA

B) (A) / Methanol=50/50


A) 100 mM HFIP + 10 mM DIPA

B) (A) / Methanol=50/50

pH Forgiveness

Explore a wide range of pH levels while reaching your optimal conditions in chromatography. FlexFire columns make this possible by maintaining high resolution between pH 1 and 10.5. View the data below to see how well FlexFire performs against our competitors!
pH Durability
Column Types Develosil FlexFire Brand A Brand B Brand C Brand D
Particle Size (µm)  1.6  1.7  1.7  1.8  1.9
Column Function Group C18
Size 2.0 x 5.0 mm
Mobile Phase Acetonitrile / 10 mM NH4OH, pH10.5=60/40
Flow Rate 0.5 mL/min
Sample Naphthalene (0.1 mg/mL)
Temperature 40°C
Detection UV 254 nm
Injection Volume 0.16 µL


Flexibility to Transfer Conditions Between HPLC and UHPLC

Save time when switching conditions between HPLC and UHPLC. Use the same mobile phase conditions while maintaining high resolution with FlexFire’s consistency in chemistry and pore size across the series.


Free application note for a guide to transfer method between HPLC and UHPLC.

FlexFire’s Metal-Free Columns

Sharpen your peaks shapes with FlexFire’s metal-free columns. Metal-free columns prevent absorption of target compounds and allow your the peaks to reach new heights!
How it Works

FlexFire’s metal-free column has a PEEK tube inside the stainless steel tube. This makes it highly durable, even under high pressure.


The PEEK interior prevents the compounds from coming into contact with the stainless steel exterior of the tube.

Metal-free diagram
In the chromatogram below, we demonstrate a comparison of results between a traditional column and a metal-free column. The metal-free column separates ATP and ADP that the traditional column did not. Additionally, the AMP shows a sharper peak shape when using the metal-free column.
Metal vs Metal-Free
Metal vs Metal-Free Conditions

FlexFire Specifications

FlexFire’s Line Up

Function Particle Size Surface Area Pore Diameter Pore Volume Carbon Load  pH Range  Temp Tolerance
C18 1.6, 2.6, and 5 µm 340 m2/g 11 nm 1.0 mL/g 22.00% 1-10 <60°C
C30 11.00%
C8 12.00%
AQ C18 8.50%
C1 5.50%
C4 mAb 2.6 and 5 µm 100 nm 1.4 mL/g 0.60% <80°C
mAb-RP 24 m2/g 100 nm 0.8 mL/g 1.30%
120 SEC  5 µm 340 m2/g 12 nm 1.0 mL/g 9.00% 2-10
300 SEC 170 m2/g 30 nm 1.4 mL/g 6.00%
WP C1 2.6 and 5 µm 3.00% 1-10
WP C4 5.00%
WP C8 7.00%
WP C18 1.6, 2.6, and 5 µm 15.00%

Pressure Tolerance

Particle Size For all Pore Sizes
1.6 µm 800 bar
2.6 µm 600 bar
5 µm 300 bar



Experience FlexFire For Yourself

You can’t appreciate FlexFire’s durability, robustness in high-pH environments, or unique ability to transfer between R&D and QC, without experiencing it firsthand.