UHPLC and HPLC Columns
Achieving New Levels of
Freedom and Performance
Maintain performance while discovering your ideal conditions with Flexfire’s wide selection of HPLC and UHPLC columns.
Superior Silica
Bring high-density, and low-metal silica to your lab. FlexFire’s superior silica is evidenced by the ability to separate up to a 1-base difference in 20-base of nucleotides.
Column: FlexFire Column mAb-RP, 2.6 µm (2.0×50 mm)
For the best resolution and retention, we suggest using Diisopropanolamine (DIPA) as your ion-pair. For comparison, we have also displayed our results while using triethylamine (TEA).
| Pore Size | 1000 Å |
| Injection Volume | 2.0 µL |
| Mixer | 100 µL |
| Sample | 20nt: 5′-GU ACG CGG AAU ACU UCG AdTdT-3′
21 nt: 5′-CGU ACG CGG AAU ACU UCG AdTdT-3′ |
| Temperature | 60°C |
| Detection | UV260 nm |
| Mobile Phase | TEA:
A) 100 mM HFIP + 10 mM TEA B) (A) / Methanol=50/50 DIPA A) 100 mM HFIP + 10 mM DIPA B) (A) / Methanol=50/50 |
pH Forgiveness
Explore a wide range of pH levels while reaching your optimal conditions in chromatography. FlexFire columns make this possible by maintaining high resolution between pH 1 and 10.5. View the data below to see how well FlexFire performs against our competitors!
| Column Types | Develosil FlexFire | Brand A | Brand B | Brand C | Brand D |
| Particle Size (µm) | 1.6 | 1.7 | 1.7 | 1.8 | 1.9 |
| Column Function Group | C18 |
| Size | 2.0 x 5.0 mm |
| Mobile Phase | Acetonitrile / 10 mM NH4OH, pH10.5=60/40 |
| Flow Rate | 0.5 mL/min |
| Sample | Naphthalene (0.1 mg/mL) |
| Temperature | 40°C |
| Detection | UV 254 nm |
| Injection Volume | 0.16 µL |
Flexibility to Transfer Conditions Between HPLC and UHPLC
Save time when switching conditions between HPLC and UHPLC. Use the same mobile phase conditions while maintaining high resolution with FlexFire’s consistency in chemistry and pore size across the series.
Free application note for a guide to transfer method between HPLC and UHPLC.
FlexFire’s Metal-Free Columns
Sharpen your peaks shapes with FlexFire’s metal-free columns. Metal-free columns prevent absorption of target compounds and allow your the peaks to reach new heights!
How it Works
FlexFire’s metal-free column has a PEEK tube inside the stainless steel tube. This makes it highly durable, even under high pressure.
The PEEK interior prevents the compounds from coming into contact with the stainless steel exterior of the tube.
In the chromatogram below, we demonstrate a comparison of results between a traditional column and a metal-free column. The metal-free column separates ATP and ADP that the traditional column did not. Additionally, the AMP shows a sharper peak shape when using the metal-free column.
FlexFire Specifications
FlexFire’s Line Up
| Function | Particle Size | Surface Area | Pore Diameter | Pore Volume | Carbon Load | pH Range | Temp Tolerance |
|---|---|---|---|---|---|---|---|
| C18 | 1.6, 2.6, and 5 µm | 340 m2/g | 11 nm | 1.0 mL/g | 22.00% | 1-10 | <60°C |
| C30 | 11.00% | ||||||
| C8 | 12.00% | ||||||
| AQ C18 | 8.50% | ||||||
| C1 | 5.50% | ||||||
| HILIC | — | ||||||
| C4 mAb | 2.6 and 5 µm | 100 nm | 1.4 mL/g | 0.60% | <80°C | ||
| mAb-RP | 24 m2/g | 100 nm | 0.8 mL/g | 1.30% | |||
| 120 SEC | 5 µm | 340 m2/g | 12 nm | 1.0 mL/g | 9.00% | 2-10 | |
| 300 SEC | 170 m2/g | 30 nm | 1.4 mL/g | 6.00% | |||
| WP C1 | 2.6 and 5 µm | 3.00% | 1-10 | ||||
| WP C4 | 5.00% | ||||||
| WP C8 | 7.00% | ||||||
| WP C18 | 1.6, 2.6, and 5 µm | 15.00% |
Pressure Tolerance
| Particle Size | For all Pore Sizes | ||
|---|---|---|---|
| 1.6 µm | 800 bar | ||
| 2.6 µm | 600 bar | ||
| 5 µm | 300 bar | ||
Brochures
Applications
Experience FlexFire For Yourself
You can’t appreciate FlexFire’s durability, robustness in high-pH environments, or unique ability to transfer between R&D and QC, without experiencing it firsthand.