FlexFire Columns - Develosil USA

UHPLC and HPLC Columns

More Powerful Conditions

Maintain performance while discovering your ideal conditions with FlexFire’s wide selection of UHPLC columns.

Superior Silica

Bring high-density, and low-metal silica to your lab. FlexFire’s superior silica is evidenced by the ability to separate up to a 1-base difference in 20-base of nucleotides.
Column: FlexFire Column mAb-RP, 2.6 µm (2.0×50 mm)

For the best resolution and retention, we suggest using Diisopropanolamine (DIPA) as your ion-pair. For comparison, we have also displayed our results while using triethylamine (TEA).

Pore Size1000 Å
Injection Volume2.0 µL
Mixer100 µL
Sample20nt: 5′-GU ACG CGG AAU ACU UCG AdTdT-3′

21 nt: 5′-CGU ACG CGG AAU ACU UCG AdTdT-3′

DetectionUV260 nm
Mobile PhaseTEA:

A) 100 mM HFIP + 10 mM TEA

B) (A) / Methanol=50/50


A) 100 mM HFIP + 10 mM DIPA

B) (A) / Methanol=50/50

pH Forgiveness

Explore a wide range of pH levels while reaching your optimal conditions in chromatography. FlexFire columns make this possible by maintaining high resolution between pH 1 and 10.5. View the data below to see how well FlexFire performs against our competitors!
pH Durability
Column TypesDevelosil FlexFireBrand ABrand BBrand CBrand D
Particle Size (µm) 1.6 1.7 1.7 1.8 1.9
Column Function GroupC18
Size2.0 x 5.0 mm
Mobile PhaseAcetonitrile / 10 mM NH4OH, pH10.5=60/40
Flow Rate0.5 mL/min
SampleNaphthalene (0.1 mg/mL)
DetectionUV 254 nm
Injection Volume0.16 µL


Flexibility to Transfer Conditions Between HPLC and UHPLC

Save time when switching conditions between HPLC and UHPLC. Use the same mobile phase conditions while maintaining high resolution with FlexFire’s consistency in chemistry and pore size across the series.


Free application note for a guide to transfer method between HPLC and UHPLC.

FlexFire’s Metal-Free Columns

Sharpen your peaks shapes with FlexFire’s metal-free columns. Metal-free columns prevent absorption of target compounds and allow your the peaks to reach new heights!
How it Works

FlexFire’s metal-free column has a PEEK tube inside the stainless steel tube. This makes it highly durable, even under high pressure.


The PEEK interior prevents the compounds from coming into contact with the stainless steel exterior of the tube.

Metal-free diagram
In the chromatogram below, we demonstrate a comparison of results between a traditional column and a metal-free column. The metal-free column separates ATP and ADP that the traditional column did not. Additionally, the AMP shows a sharper peak shape when using the metal-free column.
Metal vs Metal-Free
Metal vs Metal-Free Conditions

FlexFire Specifications

FlexFire’s Line Up

FunctionParticle SizeSurface AreaPore DiameterPore VolumeCarbon Load pH Range Temp Tolerance
C181.6, 2.6, and 5 µm340 m2/g11 nm1.0 mL/g22.00%1-10<60°C
AQ C188.50%
C4 mAb2.6 and 5 µm100 nm1.4 mL/g0.60%<80°C
mAb-RP24 m2/g100 nm0.8 mL/g1.30%
120 SEC 5 µm340 m2/g12 nm1.0 mL/g9.00%2-10
300 SEC170 m2/g30 nm1.4 mL/g6.00%
WP C12.6 and 5 µm3.00%1-10
WP C45.00%
WP C87.00%
WP C181.6, 2.6, and 5 µm15.00%

Pressure Tolerance

Particle SizeFor all Pore Sizes
1.6 µm800 bar
2.6 µm600 bar
5 µm300 bar



Experience FlexFire For Yourself

You can’t appreciate FlexFire’s durability, robustness in high-pH environments, or unique ability to transfer between R&D and QC, without experiencing it firsthand.