FlexFire Fusion - Develosil USA

More Powerful Conditions

Maintain performance while discovering your ideal conditions with FlexFire Fusion’s wide selection of HPLC columns.

FlexFire Fusion is a fusion of our latest FlexFire technology and the highest-quality aspects from our renowned, now discontinued, Develosil HPLC columns. Our new HPLC column line achieves the perfect balance between quality and affordability. This is possible due to utilizing FlexFire’s premium silica, exceptional pH tolerance, and Develosil’s newly refined particle sizes.

Reliable Results, Every Time

FlexFire Fusion introduces new and more refined particle sizes, ranging from 2 μm to 5 μm. The refined particle sizes, combined with exceedingly low impurities, ensure exceptional separation efficiency and accuracy. 

Effects of basic compounds
 USP Tailing factor
 PropranololAmitriptyline
FlexFire Fusion C181.031.09
FlexFire C181.251.39
Develosil ODS-HG1.361.73
Develosil ODS1.711.86

Conditions
ColumnFlexFire Fusion C18, 5 µm (4.6×150 mm)
FlexFire C18, 5 µm (4.6×150 mm)
Develosil ODS-HG, 5 µm (4.6×150 mm)
Develosil ODS, 5 µm (4.6×150 mm)
Mobile phase10 mM Phosphate buffer, pH 7.0 / Methanol = 30/70
Flow Rate1.0 mL/min
Temperature40 °C
DetectionUV 254 nm
Sample1. Uracil / 2. Butylparaben / 3. Propranolol /
4. Naphthalene / 5. Acenaphthene / 6. Amitriptyline
Injection Volume1.0 µL
SystemWaters Alliance

Withstand Extreme Conditions

Built to last with robust silica gel, FlexFire Fusion HPLC columns are engineered to withstand pressure of up to 1,000 bar and pH 1-11. The versatility of possible conditions lends itself to a wide range of applications while maintaining performance and longevity. 

 Fusion C30Fusion C18Fusion C18RSFusion C8Fusion Bio C4Fusion BIO C4Fusion HILIC
Pore Diammeter100 Å100 Å150 Å100 Å100 Å300 Å100 Å
Surface Area 300 m2/g300 m2/g200 m2/g300 m2/g300 m2/g120 m2/g300 m2/g
Pore Volume 0.8 L/g0.8 L/g0.8 L/g0.8 L/g0.8 L/g0.8 L/g0.8 L/g
Carbon (%) 17%18%12%12%8%4%
Particle Size (µm)2, 3, 5 µm2, 3, 5 µm2, 3, 5 µm2, 3, 5 µm2, 3, 5 µm2, 3, 5 µm2, 3, 5 µm
pH Rangeph 1-11ph 1-11ph 1-11ph 1-11ph 1-10ph 1-10ph 1-7
Temp. range (°C)~80 °C~80 °C~80 °C~80 °C~80 °C~80 °C~80 °C
Max Pressure (bar)

2 µm: 1000 bar

3 µm: 600 bar

5 µm: 400 bar

2 µm: 1000 bar

3 µm: 600 bar

5 µm: 400 bar

2 µm: 1000 bar

3 µm: 600 bar

5 µm: 400 bar

2 µm: 1000 bar

3 µm: 600 bar

5 µm: 400 bar

2 µm: 1000 bar

3 µm: 600 bar

5 µm: 400 bar

2 µm: 1000 bar

3 µm: 600 bar

5 µm: 400 bar

2 µm: 1000 bar

3 µm: 600 bar

5 µm: 400 bar

USP CategoryL62L1L1L7L26L26L3

High Performance, Low Cost

FlexFire Fusion found the perfect balance between cost and performance. This was made possible by the combination of our best performing Develosil HPLC columns and innovative manufacturing technology introduced with the FlexFire UHPLC column series.

Chromatogram FlexFire Fusion

Conditions
Column

FlexFire Fusion C30, 5 µm (4.6 x 150 mm)

FlexFire Fusion C18, 5 µm (4.6 x 150 mm)

FlexFire Fusion C18RS, 5 µm (4.6 x 150 mm)

FlexFire Fusion C8, 5 µm (4.6 x 150 mm)

FlexFire Fusion C4, 5 µm (4.6 x 150 mm)

Mobile phaseWater / Methanol = 60/40
Flow Rate1.0 mL/min
Temperature40 ℃
DetectionUV 365 nm
Sample

1. Aflatoxin G2 (25 µg/mL) 2. Aflatoxin G1 (25 µg/mL)

3. Aflatoxin B2 (25 µg/mL) 4. Aflatoxin B1 (25 µg/mL)

Injection Volume5.0 µL
SystemWaters Arc HPLC

FlexFire Fusion’s Metal-Free Columns

Elevate your chromatographic results with FlexFire’s recommended upgrade to metal-free columns. Experience sharpened peak shapes, as these advanced columns prevent target compound absorption, allowing your peaks to achieve unprecedented heights.

How it Works

FlexFire’s innovative metal-free column design features a PEEK tube within the stainless steel exterior. This construction allows remarkable durability, even when operating under high-pressure conditions.

 

The PEEK interior of our metal-free columns prevent compounds from interacting with the stainless steel surface, avoiding unwanted adsorption and peak distortion. This ensures high-resolution separations and accurate results in LC and HPLC analyses.

Metal-free diagram

In the chromatogram below, we compare a traditional HPLC column with a metal-free HPLC column. The metal-free column effectively separates ATP and ADP, which the traditional column fails to achieve. Furthermore, the metal-free column produces a sharper peak shape for AMP.

Metal vs Metal-Free
Metal vs Metal-Free Conditions

Upgrading from Develosil to FlexFire Fusion

When transitioning from a Develosil Series column to a FlexFire or FlexFire Fusion Series column, it is of utmost importance to choose the correct column. In order to aid in this process, please refer to the table below comparing our discontinued Develosil Series columns to their corresponding FlexFire and FlexFire Fusion Series columns.

Develosil SeriesFlexFire (UHPLC)FlexFire Fusion (HPLC)
  • ODS
  • ODS-K
  • ODS-N
  • ODS-T
  • ODS-HG
  • ODS-UG
FlexFire C18Fusion C18
ODS-PFusion C18RS
ODS-MGFlexFire AQ C18
  • TMS
  • TMS-UG
FlexFire C1
  • C8
  • C8-UG
FlexFire C8Fusion C8
  • RPAQUEOUS (C30-UG)
  • RPAQUEOUS-AR
FlexFire C30Fusion C30
100 DiolFlexFire 120SEC
300DiolFlexFire 300SEC
  • 300 ODS-HG
  • 300 ODS-UG
  • 300 C8-HG
  • 300 C4-HG
  • FlexFire WP C18
  • FlexFire WP C8
  • FlexFire WP C4
  • FlexFire WP C1
Fusion Bio C4
Silica 100FlexFire HILICFusion HILIC
  • ODS-A
  • ODS-SR
  • PAHS
  • Ph
  • Ph-UG
  • CN
  • CN-UG
  • NH2
  • XG-C18M
  • XG-C18LC
  • XG-C30M
  • XG-C1
  • XG-CN
  • HSR C18
  • HSR AQ C18
  • HSR C18Peptide
  • HSR-C1
No equivalent ProductsNo equivalent Products

 

Experience FlexFire Fusion HPLC Columns For Yourself

Experience reliable and accurate results with FlexFire Fusion’s innovative HPLC columns, offering refined particle sizes and exceptional separation efficiency. Upgrade to our recommended metal-free columns to prevent unwanted interactions and achieve peak precision. Claim your demo column now and discover the perfect balance of high performance and cost-effectiveness for your chromatographic needs.