Perfected HPLC Columns
Showing performance and durability in highly demanding conditions
FlexFire Fusion is a fusion of our latest FlexFire technology and the highest-quality aspects from our renowned, now discontinued, Develosil HPLC columns. Our new HPLC column line achieves the the perfect balance between quality and affordability. This is possible due to utlilizing FlexFire’s premium silica, exceptional pH tolerance, and Develosil’s newly refined particle sizes.
Reliable Results, Every Time
FlexFire Fusion introduces new and more refined particle sizes, ranging from 2 μm to 5 μm. The refined particle sizes, combined with exceedingly low impurities, ensure exceptional separation efficiency and accuracy.
| Effects of basic compounds | |||
| USP Tailing factor | |||
| Propranolol | Amitriptyline | ||
| FlexFire Fusion C18 | 1.03 | 1.09 | |
| FlexFire C18 | 1.25 | 1.39 | |
| Develosil ODS-HG | 1.36 | 1.73 | |
| Develosil ODS | 1.71 | 1.86 | |
| Conditions | |
| Column | FlexFire Fusion C18, 5um (4.6x150mm) FlexFire C18, 5um (4.6x150mm) Develosil ODS-HG, 5um (4.6x150mm) Develosil ODS, 5um (4.6x150mm) |
| Mobile phase | 10 mM Phosphate buffer, pH 7.0 / Methanol = 30/70 |
| Flow Rate | 1.0 mL/min |
| Temperature | 40 ℃ |
| Detection | UV 254 nm |
| Sample | 1. Uracil ／ 2. Butylparaben ／ 3. Propranolol ／ 4. Naphthalene ／ 5. Acenaphthene ／ 6. Amitriptyline |
| Injection Volume | 1.0 µL |
| System | Waters Alliance |
Withstand Extreme Conditions
Built to last with robust silica gel, FlexFire Fusion HPLC columns are engineered to withstand pressure of up to 1,000 bar and pH 1-11. The versatility of possible conditions lends itself to a wide range of applications while maintaining performance and longevity.
| Fusion C30 | Fusion C18 | Fusion C18RS | Fusion C8 | Fusion Bio C4 | Fusion BIO C4 | Fusion HILIC | |
| Pore Diammeter | 100 Å | 100 Å | 150 Å | 100 Å | 100 Å | 300 Å | 100 Å |
| Surface Area | 300 m2/g | 300 m2/g | 200 m2/g | 300 m2/g | 300 m2/g | 120 m2/g | 300 m2/g |
| Pore Volume | 0.8 L/g | 0.8 L/g | 0.8 L/g | 0.8 L/g | 0.8 L/g | 0.8 L/g | 0.8 L/g |
| Carbon (%) | 17% | 18% | 12% | 12% | 8% | 4% | – |
| Particle Size (µm) | 2, 3, 5 µm | 2, 3, 5 µm | 2, 3, 5 µm | 2, 3, 5 µm | 2, 3, 5 µm | 2, 3, 5 µm | 2, 3, 5 µm |
| pH Range | ph 1-11 | ph 1-11 | ph 1-11 | ph 1-11 | ph 1-10 | ph 1-10 | ph 1-7 |
| Temp. range (°C) | ~80 °C | ~80 °C | ~80 °C | ~80 °C | ~80 °C | ~80 °C | ~80 °C |
| Max Pressure (bar) |
2 µm: 1000 bar 3 µm: 600 bar 5 µm: 400 bar |
2 µm: 1000 bar 3 µm: 600 bar 5 µm: 400 bar |
2 µm: 1000 bar 3 µm: 600 bar 5 µm: 400 bar |
2 µm: 1000 bar 3 µm: 600 bar 5 µm: 400 bar |
2 µm: 1000 bar 3 µm: 600 bar 5 µm: 400 bar |
2 µm: 1000 bar 3 µm: 600 bar 5 µm: 400 bar |
2 µm: 1000 bar 3 µm: 600 bar 5 µm: 400 bar |
| USP Category | L62 | L1 | L1 | L7 | L26 | L26 | L3 |
High Performance, Low Cost
FlexFire Fusion found the perfect balance between cost and performance. This was made possible by the combination of our best performing Develosil HPLC columns and innovative manufacturing technology introduced with the FlexFire UHPLC column series.
| Conditions | |
| Column |
FlexFire Fusion C30, 5 µm (4.6 x 150 mm) FlexFire Fusion C18, 5 µm (4.6 x 150 mm) FlexFire Fusion C18RS, 5 µm (4.6 x 150 mm) FlexFire Fusion C8, 5 µm (4.6 x 150 mm) FlexFire Fusion C4, 5 µm (4.6 x 150 mm) |
| Mobile phase | Water / Methanol = 60/40 |
| Flow Rate | 1.0 mL/min |
| Temperature | 40 ℃ |
| Detection | UV 365 nm |
| Sample |
1. Aflatoxin G2 (25 µg/mL) 2. Aflatoxin G1 (25 µg/mL) 3. Aflatoxin B2 (25 µg/mL) 4. Aflatoxin B1 (25 µg/mL) |
| Injection Volume | 5.0 µL |
| System | Waters Arc HPLC |
FlexFire Fusion’s Metal-Free Columns
Elevate your chromatographic results with FlexFire’s recommended upgrade to metal-free columns. Experience sharpened peak shapes, as these advanced columns prevent target compound absorption, allowing your peaks to achieve unprecedented heights.
How it Works
FlexFire’s innovative metal-free column design features a PEEK tube within the stainless steel exterior. This construction allows remarkable durability, even when operating under high-pressure conditions.
The PEEK interior of our metal-free columns prevent compounds from interacting with the stainless steel surface, avoiding unwanted adsorption and peak distortion. This ensures high-resolution separations and accurate results in LC and HPLC analyses.
In the chromatogram below, we compare a traditional HPLC column with a metal-free HPLC column. The metal-free column effectively separates ATP and ADP, which the traditional column fails to achieve. Furthermore, the metal-free column produces a sharper peak shape for AMP.
Upgrading from Develosil to FlexFire Fusion
When transitioning from a Develosil Series column to a FlexFire or FlexFire Fusion Series column, it is of utmost importance to choose the correct column. In order to aid in this process, please refer to the table below comparing our discontinued Develosil Series columns to their corresponding FlexFire and FlexFire Fusion Series columns.
| Develosil Series | FlexFire (UHPLC) | FlexFire Fusion (HPLC) |
|---|---|---|
|
FlexFire C18 | Fusion C18 |
| ODS-P | Fusion C18RS | |
| ODS-MG | FlexFire AQ C18 | |
|
FlexFire C1 | |
|
FlexFire C8 | Fusion C8 |
|
FlexFire C30 | Fusion C30 |
| 100 Diol | FlexFire 120SEC | |
| 300Diol | FlexFire 300SEC | |
|
|
Fusion Bio C4 |
| Silica 100 | FlexFire HILIC | Fusion HILIC |
|
No equivalent Products | No equivalent Products |
Experience FlexFire Fusion HPLC Columns For Yourself
Experience reliable and accurate results with FlexFire Fusion’s innovative HPLC columns, offering refined particle sizes and exceptional separation efficiency. Upgrade to our recommended metal-free columns to prevent unwanted interactions and achieve peak precision. Claim your free demo column now and discover the perfect balance of high performance and cost-effectiveness for your chromatographic needs.