Analysis of Xanthine Derivaties using HPLC-UV - Develosil USA

Analysis of Xanthine Derivatives using HPLC-UV

Analysis of Xanthine Derivatives using HPLC-UV

Sample:

  1. Xanthine (10 µg/mL)
  2. Theobromine (18 µg/mL)
  3. Theophiline (21 µg/mL)
  4. Caffeine (38 µg/mL)

HPLC Analytical Conditions:

Column:  Develosil HSR C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase: Methanol/Water=30/70
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 254 nm
Injection Volume: 20 µL

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