Analysis of Xanthine Derivatives using HPLC-UV
Sample:
- Xanthine (10 µg/mL)
- Theobromine (18 µg/mL)
- Theophiline (21 µg/mL)
- Caffeine (38 µg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil HSR C18, 5 µm ( 4.6 x 150 mm) |
| Mobile Phase: | Methanol/Water=30/70 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 254 nm |
| Injection Volume: | 20 µL |
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