Analysis of Water-soluble Vitamins
using HPLC-UV (Part 1)
Sample:
- Thiamine (0.5 mg/mL)
- Nicotineamide (1.0 mg/mL)
- Riboflavin (1.0 mg/mL)
- Cyanocobalamin (1.0 mg/mL)
- Nicotinic acid (1.0 mg/mL)
- Ascorbic acid (3.0 mg/mL)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil ANIDIUS (4.6 x 250 mm) |
| Mobile Phase: | A) Acetonitrile+0.1% Formic Acid B) 0.1 Formic Acid |
| Gradient: | B) 25% – 60% (15 min) ➞ 60% (30 min) |
| Flow Rate: | 0.5 mL/min |
| Temperature: | 40°C |
| Detection: | UV260 nm |
| Injection Volume: | 2.0 µL |
Similar Applications:
Water-soluble Vitamins (Part 2)
Water-soluble Vitamins (Part 3)
Water-soluble Vitamins (Part 4)
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