Analysis of Water-Soluble Vitamins
using HPLC-UV (Part 3)
Sample:
- L(+)-Ascorbic acid (53 µg/mL)
- Nicotinic acid (17 µg/mL)
- Nicotinamide (15 µg/mL)
- Calcium (+)-pantothenate (0.41 mg/mL)
- Pyridoxine hydrochloride (17 µg/mL)
- Riboflavin sodium phosphate (53 µg/mL)
- Folic acid (21 µg/mL)
- (+)-Biotin (0.15 mg/mL)
- Tiamin hydrochloride (0.10 mg/mL)
- Cyanocobalamin (52 µg/mL)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil HSR C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | A) 20 mM NH₄H₂PO₄ (pH=3.0) contianing 5 mM CH₃(CH₂)₅SO₃Na B) Methanol containing 5 mM CH₃(CH₂)₅SO₃Na |
| Gradient: | B) 5% (0-5 min), 5%-25% (5-25 min), 25% (25-35 min) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 210 nm |
| Injection Volume: | 5 µL |
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