Analysis of Water-soluble Vitamins
using HPLC-UV (Part 2)
Sample:
- L(+)-Ascorbic acid (0.04 mg/mL)
- Orotic acid (0.09 mg/mL)
- Nicotinic acid (0.18 mg/mL)
- Pyridoxine HCl (0.23 mg/mL)
- Nicotinamide (0.22 mg/mL)
- Folic acid (0.22 mg/mL)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil XG-C18M, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | Acetonitrile/50 mM Ammonium Formate (2/98) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 30°C |
| Detection: | UV at 260 nm |
| Injection Volume: | 2.0 µL |
Similar Applications:
Water-soluble Vitamins (Part 1)
Water-soluble Vitamins (Part 3)
Water-soluble Vitamins (Part 4)
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