Analysis of Vitamins using the Ion Pair Method
Sample:
- Ascorbic acid (0.12 mg/mL)
- Nicotinamide (0.12 mg/mL)
- Nicotinic acid (0.11 mg/mL)
- Thiamin Hydrochloride (0.13 mg/mL)
- Pyridoxine Hydrochloride (0.12 mg/mL)
- Calcium (+)-Pantothenate (1.14 mg/mL)
- Folic acid (0.12 mg/mL)
- Biotin (0.61 mg/mL)
- Cyanocobalamin (0.11 mg/mL)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | 1. Develosil XG-C18LC, 5 µm (4.6 x 150 mm) 2. Develosil XG-C18M, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | A) 0.1% TFA in Water B) 0.1% TFA in Methanol |
| Gradient: | B) 0%-70% (0-20 min) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 210 nm |
| Injection Volume: | 5.0 µL |
Download the product brochure or browse our technical library to find additional applications and publications.