Analysis of Sulfa Drugs using UHPLC-UV
Sample:
- Sulfadiazine (0.08 mg/mL)
- Sulfathiazole (0.10 mg/mL)
- Sulfapyridine (0.10 mg/mL)
- Sulfamerazine (0.09 mg/mL)
- Sulfamethazine (0.09 mg/mL)
- Sulfamethoxypyridazine (0.09 mg/mL)
- Sulfachloropyridazine (0.10 mg/mL)
- Sulfamethoxazole (0.10 mg/mL)
- Sulfadimethoxine (0.10 mg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil HSR AQ C18, 2 µm ( 2.0 x 50 mm) |
| Mobile Phase: | A) Water + 0.1% HCOOH B) Acetonitrile + 0.1% HCOOH |
| Gradient: | Further details here |
| Flow Rate: | 0.474 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 275 nm |
| Injection Volume: | 0.16 µL |
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