Analysis of Sulfa Drugs using UHPLC-UV

Sample:
- Sulfadiazine (0.08 mg/mL)
- Sulfathiazole (0.10 mg/mL)
- Sulfapyridine (0.10 mg/mL)
- Sulfamerazine (0.09 mg/mL)
- Sulfamethazine (0.09 mg/mL)
- Sulfamethoxypyridazine (0.09 mg/mL)
- Sulfachloropyridazine (0.10 mg/mL)
- Sulfamethoxazole (0.10 mg/mL)
- Sulfadimethoxine (0.10 mg/mL)
HPLC Analytical Conditions: |
|
---|---|
Column: | Develosil HSR AQ C18, 2 µm ( 2.0 x 50 mm) |
Mobile Phase: | A) Water + 0.1% HCOOH B) Acetonitrile + 0.1% HCOOH |
Gradient: | Further details here |
Flow Rate: | 0.474 mL/min |
Temperature: | 40°C |
Detection: | UV at 275 nm |
Injection Volume: | 0.16 µL |
Download the product brochure or browse our technical library to find additional applications and publications.