Analysis of Sulfa Drugs using HPLC-UV
Sample:
- Sulfadiazine (0.04 mg/mL)
- Sulfathiazole (0.05 mg/mL)
- Sulfapyridine (0.06 mg/mL)
- Sulfamerazine (0.06 mg/mL)
- Sulfamethazine (0.06 mg/mL)
- Sulfamethoxypyridazine (0.06 mg/mL)
- Sulfachloropyridazine (0.05 mg/mL)
- Sulfamethoxazole (0.05 mg/mL)
- Sulfamethoxine (0.08 mg/mL)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | 1. Develosil HSR C18, 5 µm ( 4.6 x 150 mm) 2. Develosil HSR C18, 3 µm (4.6 x 100 mm) |
| Mobile Phase: | A) Water + 0.1% HCOOH B) Methanol + 0.1% HCOOH |
| Gradient: | Further details here |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 275 nm |
| Injection Volume: | 2.0 µL |
Download the product brochure or browse our technical library to find additional applications and publications.