Analysis of Sinigrin using HPLC-UV
Sample:
Sinigirin (0.1 mg/mL)
| HPLC Analytical Conditions: | |
|---|---|
| Column: | 1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm) 2. Develosil HSR C1, 5 µm (4.6 x 150 mm) 3. Develosil C30-UG, 5 µm (4.6 x 150 mm) 4. Develosil ODS-HG, 5 µm (4.6 x 150 mm) 5. Develosil ODS, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | 10 mM Monopotassium Phosphate (pH=2.0) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 210 nm |
| Injection Volume: | 2.0 µL |
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