Analysis of Retinol (Vitamin A) using HPLC-UV
Sample:
- Retinoic Acid (68 µg/mL)
- Retinol (55 µg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil HSR C18, 5 µm ( 4.6 x 150 mm) |
| Mobile Phase: | Acetonitrile/Water=95/5 |
| Flow Rate: | 0.5 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 325 nm |
| Injection Volume: | 2.0 µL |
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