Analysis of Resveratrol in Red Wine using HPLC-UV

Sample:
- Resveratrol Standard (0.58 µg/mL)
- Red Wine
Red Wine Sample Preparation:
Red wine was filtered through a 0.45 µm filter.
HPLC Analytical Conditions: |
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Column: | Develosil HSR C18, 5 µm ( 4.6 x 150 mm) |
Mobile Phase: | Acetonitrile/Water=20/80 |
Flow Rate: | 1.0 mL/min |
Temperature: | 40°C |
Detection: | UV at 280 nm |
Injection Volume: | 1. Resveratrol Standard (5 µL) 2. Red Wine (10 µL) |
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