Analysis of PTH-Amino Acids using HPLC-UV
Sample:
- PTH-Histidine
- PTH-Arginine
- PTH-Asparagine
- PTH-Glutamine
- PTH-Aspartic acid
- PTH-Glycine
- PTH-Glutamic acid
- PTH-Alamine
- PTH-Tyrosine
- PTH-Threonine
- PTH-Methionine
- PTH-Valine
- PTH-Proline
- PTH-Tryptophan
- PTH-Lysine
- PTH-Phenylalanine
- PTH-Isoeucine
- PTH-Leucine
**Each sample concentration is 10 nmol/mL
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil HSR C18, 5 µm (4.6 x 250 mm) |
| Mobile Phase: | A) 25 mM NH₄H₂PO₄ (pH=2.0) B) Acetonitrile |
| Gradient | See more details here. |
| Flow Rate: | 0.7 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 254 nm |
| Mixer Volume: | 10 µL |
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