Analysis of Peptide using HPLC
Sample:
- Gly-Tyr (0.05 mg/mL)
- Val-Tyr-Val (0.05 mg/mL)
- Met-Enkephalin (0.05 mg/mL)
- Angiotensin II (0.05 mg/mL)
- Leu-Enkephalin (0.05 mg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | 1. Develosil HSR C1, 5 µm (4.6 x150 mm) 2. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | A) 25 mM NaCl (pH=3.0) B) Acetonitrile |
| Gradient: | Further details here |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Mixer Volume: | 10 µL |
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