Analysis of Peptide using UHPLC
Sample:
- Gly-Tyr (0.02 mg/mL)
- Val-Tyr-Val (0.02 mg/mL)
- Met-Enkephalin (0.02 mg/mL)
- Anggiontensin II (0.002 mg/mL)
- Leu-Enkephalin (0.02 mg/mL)
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HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil HSR AQ C18, 2 µm ( 2.0 x 50 mm) |
| Mobile Phase: | A) Water +0.1% TFA B) Acetonitrile + 0.1% TFA |
| Gradient: | Further details here |
| Flow Rate: | 0.474 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 230 nm |
| Injection Volume: | 0.16 µL |
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