Analysis of Nucleobases using HPLC-UV | Develosil USA

Analysis of Nucleobases using HPLC-UV

Analysis of Nucleobases using HPLC-UV

Sample:

  1. Cytosine (0.38 mg/mL)
  2. Uracil (0.36 mg/mL)
  3. Cytidine (0.36 mg/mL)
  4. Uridine (0.38 mg/mL)
  5. Thymine (0.39 mg/mL)

HPLC Analytical Conditions:

Column:  Develosil HSR C18, 5 µm ( 4.6 x 250 mm)
Mobile Phase: Methanol/25 mM Diammonium hydrogen phosphate=5/95
Flow Rate: 0.5 mL/min
Temperature: 40°C
Detection: UV at 254 nm
Injection Volume: 2.0 µL

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