Analysis of Nucleobases using HPLC-UV
Sample:
- Cytosine (0.38 mg/mL)
- Uracil (0.36 mg/mL)
- Cytidine (0.36 mg/mL)
- Uridine (0.38 mg/mL)
- Thymine (0.39 mg/mL)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil HSR C18, 5 µm ( 4.6 x 250 mm) |
| Mobile Phase: | Methanol/25 mM Diammonium hydrogen phosphate=5/95 |
| Flow Rate: | 0.5 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 254 nm |
| Injection Volume: | 2.0 µL |
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