Analysis of Nucleic Acid
Related Compounds using HPLC-UV
Sample:
- Cytosine (0.05 mg/mL)
- Uracil (0.05 mg/mL)
- Cytidine (0.05 mg/mL)
- Guanine (0.08 mg/mL)
- Uridine (0.06 mg/mL)
- Thymine (0.05 mg/mL)
- Adenine (0.06 mg/mL)
- Guanosine (0.06 mg/mL)
- Thymidine (0.06 mg/mL)
- Adenosine (0.05 mg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | 1. Develosil HSR AQ C18, 5 µm (4.6×150 mm) 2. Develosil HSR AQ C18, 3 µm (4.6×75 mm) |
| Mobile Phase: | A) 50 mM HCOONH₄ (pH=7.0) B) Acetonitrile |
| Gradient: | Further details here |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 30°C |
| Detection: | UV at 254 nm |
| Injection Volume: | 2.0 µL |
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