Analysis of Metformin Hydrochloride | Develosil USA

Analysis of Metformin Hydrochloride using HPLC-UV

Analysis of Metformin Hydrochloride using HPLC-UV

Sample:

  1. Metformin HCl
  2. p-Hydroxybenzoic acid isobutyl ester

HPLC Analytical Conditions:

Column:  Develosil HSR AQ C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase Preparation: 1. Dissolve 0.8 g sodium lauryl sulfate in 620 mL of diluted phosphoric acid (1–>2500)
2. Add 380 mL Acetonitrile
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 235 nm
Injection Volume: 5.0 µL

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