Analysis of Metformin Hydrochloride using HPLC-UV
Sample:
- Metformin HCl
- p-Hydroxybenzoic acid isobutyl ester
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil HSR AQ C18, 5 µm ( 4.6 x 150 mm) |
| Mobile Phase Preparation: | 1. Dissolve 0.8 g sodium lauryl sulfate in 620 mL of diluted phosphoric acid (1–>2500) 2. Add 380 mL Acetonitrile |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 235 nm |
| Injection Volume: | 5.0 µL |
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