Analysis of Isoflavones using HPLC-UV - Develosil USA

Analysis of Isoflavones using HPLC-UV

Analysis of Isoflavones of using HPLC-UV


  1. Puerarin (0.31 mg/mL)
  2. Daidzin (0.31 mg/mL)
  3. Daidzein (0.33 mg/mL)
  4. Biochanin A (0.28 mg/mL)
  5. Ipriflavone (0.28 mg/mL)

HPLC Analytical Conditions:

Column: Develosil XG-C18LC, 5 µm ( 4.6 x 150 mm)
Mobile Phase:A) 0.2% Formc Acid-Water
B)  0.2% Formic Acid- Aceteonitrile
Gradient:Further details here
Flow Rate:1.0 mL/min
Detection:UV at 254 nm
Injection Volume:10 µL

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