Analysis of Isoflavones using HPLC-UV
Sample:
- Puerarin (0.31 mg/mL)
- Daidzin (0.31 mg/mL)
- Daidzein (0.33 mg/mL)
- Biochanin A (0.28 mg/mL)
- Ipriflavone (0.28 mg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil XG-C18LC, 5 µm ( 4.6 x 150 mm) |
| Mobile Phase: | A) 0.2% Formc Acid-Water B) 0.2% Formic Acid- Aceteonitrile |
| Gradient: | Further details here |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 254 nm |
| Injection Volume: | 10 µL |
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