Analysis of Food Additives in a Soft Drink using HPLC-UV
Sample:
- Acesulfame K (0.50 mg/mL)
- Sodium Benzoate (0.49 mg/ml)
- Sodium Saccharin Dihydrate (0.54 mg/ml)
- Potassium Sorbate (0.45 mg/mL)
- Caffeine (0.46 mg/mL)
- Aspartame (0.46 mg/mL)
| HPLC Analytical Conditions: | |
|---|---|
| Column: | 1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm) 2. Develosil HSR C18, 5 µm (4.6 x 150 mm) 3. Develosil HSR C1, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | A) 10 mM Diammonium Phosphate (pH 6.0) B) Acetonitrile |
| Gradient: | B) 10%-50% (0-10 min) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 214 nm |
| Injection Volume: | 1.0 µL |
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