Analysis of Flavonoids using HPLC-UV

Sample:
- Puearin (0.025 mg/mL)
- Daidzein (0.025 mg/mL)
- Biochanin A (0.027 mg/mL)
- Ipriflavone (0.025 mg/mL)
HPLC Analytical Conditions: |
|
---|---|
Column: | Develosil HSR AQ C18, 3 µm ( 2.0 x 50 mm) |
Mobile Phase: | A) 0.2% HCOOH in Water B) 0.2% HCOOH in Acetonitrike |
Gradient: | Further details here |
Flow Rate: | 0.35 mL/min |
Temperature: | 40°C |
Detection: | UV at 254 nm |
Injection Volume: | 1.0 µL |
Download the product brochure or browse our technical library to find additional applications and publications.