Analysis of Flavonoids using HPLC-UV
Sample:
- Puearin (0.025 mg/mL)
- Daidzein (0.025 mg/mL)
- Biochanin A (0.027 mg/mL)
- Ipriflavone (0.025 mg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil HSR AQ C18, 3 µm ( 2.0 x 50 mm) |
| Mobile Phase: | A) 0.2% HCOOH in Water B) 0.2% HCOOH in Acetonitrike |
| Gradient: | Further details here |
| Flow Rate: | 0.35 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 254 nm |
| Injection Volume: | 1.0 µL |
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