Analysis of Fatty Acids using HPLC-UV
Sample:
- Lauric acid (0.04 mg/mL)
- Linolenic acid (0.05 mg/mL)
- Myristic acid (0.04 mg/mL)
- Linoleic acid (0.07 mg/mL)
- Oleic acid (0.05 mg/mL)
- Stearic acid (0.04 mg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil HSR C18, 5 µm (4.6 x 150 mm) Develosil HSR C18, 3 µm (4.6 x 150 mm) |
| Mobile Phase: | Acetonitrile/25 mM NH₄H₂PO₄ (pH=2.0) = 80/20 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 400 nm |
| Injection Volume: | 10 µL |
Fatty Acids Sample Preparation:
Fatty acids were derivatized with
2-NPH (YMC Co., Ltd)
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