Analysis of Common Ingredients
in Cold Medicines using HPLC-UV
Sample:
- Acetaminophen (0.13 mg/mL)
- Caffeine (0.03 mg/mL)
- Salicylamide (0.14 mg/mL)
- Aspirin (0.58 mg/mL)
- Ethenzamide (0.28 mg/mL)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil HSR C18, 5 µm ( 4.6 x 150 mm) |
| Mobile Phase: | Methanol/50 mM NH₄H₂PO₄ (pH=2.5) = 40/60 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 280 nm |
| Injection Volume: | 5.0 µL |
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