Analysis of dNTP using UHPLC-UV
Sample:
- dCTP (0.5 mM)
- dTTP (0.5 mM)
- dGTP (0.5 mM)
- dATP (0.5 mM)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil HSR AQ C18, 2 µm (2.0 x 50 mm) |
| Mobile Phase: | 25 mM Ammonium Phosphate (pH=7.0) |
| Flow Rate: | 0.474 mL/min |
| Temperature: | 40°C (Forced Air) |
| Detection: | UV at 254 nm |
| Injection Volume: | 0.08 µL |
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