Analysis of Digested Protein using HPLC-UV

Sample:
- Insulin
- BSA
- Mab
- Thyroglobulin
Protein Digestion:
- Weight 1.0 to 2.0 mg each of insulin, BSA, monoclonal antibody, and thyroglobulin.
- Add 1.0 mL of SMART digest buffer and stir thoroughly.
- Place 200 µL of the above sample in a SMART digest tube and mix at a T=70°C, 1,400 RPM 2 hours using Thermomixer C.
- Centrifuge the prepared, mixed samples for injection.
The “SMART Digest kit” used in this research is a registered product of Thermo Fisher SCIENTIFIC.
The “Thermomixer C” used in this research is a registered product of Eppendorf.
HPLC Analytical Conditions: |
|
---|---|
Column: | Develosil HSR C18 Peptide, 3 µm ( 4.6 x 75 mm) |
Mobile Phase: | A) Water + 0.1% TFA B) Acetonitrile +0.1% TFA |
Gradient: | Further details here |
Flow Rate: | 1.0 mL/min |
Temperature: | 50°C |
Detection: | UV at 210 nm |
Injection Volume: | (1) 10 µL (2)-(4) 20 µL |
Download the product brochure or browse our technical library to find additional applications and publications.