Analysis of Digested Protein using HPLC-UV
- Weight 1.0 to 2.0 mg each of insulin, BSA, monoclonal antibody, and thyroglobulin.
- Add 1.0 mL of SMART digest buffer and stir thoroughly.
- Place 200 µL of the above sample in a SMART digest tube and mix at a T=70°C, 1,400 RPM 2 hours using Thermomixer C.
- Centrifuge the prepared, mixed samples for injection.
The “SMART Digest kit” used in this research is a registered product of Thermo Fisher SCIENTIFIC.
The “Thermomixer C” used in this research is a registered product of Eppendorf.