Fatty Acid Analysis using HPLC-PDA
Sample:
- Lauric acid (2.0 mg/mL)
- Linolenic acid (0.1 µL/mL)
- Myristic acid (4.0 mg/mL)
- Linoleic acid (0.2 µL/mL)
- Oleic acid (2.0 µL/mL)
- Stearic acid (2.1 mg/mL)
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HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil XG-C18M 5 µm ( 4.6 x 150 mm) |
| Mobile Phase: | Acetonitrile/0.2%Phosphoric acid=80/20 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 210 nm (PDA) |
| Injection Volume: | 10 µL |
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