Analysis of Catecholamine using HPLC-UV | Develosil USA

Analysis of Catecholamines using HPLC-UV

Analysis of Catecholamines using HPLC-UV

Sample:

  1. (R)-(-) Norepinephrine (0.1 mg/mL)
  2. (R)-(-) Epinephrine (0.1 mg/mL)
  3. Dopamine (0.1 mg/mL)

HPLC Analytical Conditions:

Column:  1. Develosil XG-C1, 5 µm (4.6 x 150 mm)
2. Develosil XG-C18LC, 5 µm (4.6 x 150 mm)
3. Develosil XG-C18M, 5 µm (4.6 x 150 mm)
4. Develosil XG-C30, 5 µm (4.6 x 150 mm)
Mobile Phase: 0.1M KH₂PO₄ (pH=3.0
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 280 nm
Injection Volume: 2.0 µL

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