Analysis of Catecholamines using HPLC-UV
Sample:
- (R)-(-) Norepinephrine (0.1 mg/mL)
- (R)-(-) Epinephrine (0.1 mg/mL)
- Dopamine (0.1 mg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | 1. Develosil XG-C1, 5 µm (4.6 x 150 mm) 2. Develosil XG-C18LC, 5 µm (4.6 x 150 mm) 3. Develosil XG-C18M, 5 µm (4.6 x 150 mm) 4. Develosil XG-C30, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | 0.1M KH₂PO₄ (pH=3.0 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 280 nm |
| Injection Volume: | 2.0 µL |
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