Analysis of Carotenes in Carrots using HPLC-UV
Sample:
- α-carotene
- ß-carotene
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil HSR C18, 5 µm ( 4.6 x 250 mm) |
| Mobile Phase: | Methanol/Ethanol=90/10 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 450 nm |
| Injection Volume: | 10.0 µL |
Carrot Sample Preparation:
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