Analysis of Ascorbic Acid using HPLC-CAD
Sample:
- Dehydroascorbic acid (0.34 mg/mL)
- L(+)-Ascorbic acid (0.34 mg/mL)
- Isoascorbic acid (0.34 mg/mL)
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil XG-C30M, 5 µm ( 4.6 x 150 mm) |
| Mobile Phase: | 20 mM Ammonium Formate (pH=2.0) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | CAD |
| Injection Volume: | 2.0 µL |
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