Analysis of Ascorbic Acid using HPLC-CAD

Sample:
- Dehydroascorbic acid (0.34 mg/mL)
- L(+)-Ascorbic acid (0.34 mg/mL)
- Isoascorbic acid (0.34 mg/mL)
HPLC Analytical Conditions: | |
---|---|
Column: | Develosil XG-C30M, 5 µm ( 4.6 x 150 mm) |
Mobile Phase: | 20 mM Ammonium Formate (pH=2.0) |
Flow Rate: | 1.0 mL/min |
Temperature: | 40°C |
Detection: | CAD |
Injection Volume: | 2.0 µL |
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