Analysis of Antiepileptics using HPLC-UV
Sample:
- Primidone (0.05 mg/mL)
- Ethosuximide (0.76 mg/mL)
- Carbamazepine (0.04 mg/mL)
- Phenytoin (0.08 mg/mL)
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HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil HSR C18, 5 µm ( 4.6 x 150 mm) |
| Mobile Phase: | Fig. 1: Acetonitrile/25 mM NH₄H₂PO₄ (pH=6.7)=32/68 Fig. 2: Methanol/25 mM NH₄H₂PO₄ (pH 6.7)=50/50 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 220 nm |
| Injection Volume: | 5.0 µL |
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