Analysis of 6 Acidic Compounds using HPLC-UV
Sample:
- Oxalic Acid (0.16 mg/mL)
- L(+)–Ascorbic acid (0.80 mg/mL)
- Maleic acid (0.08 mg/mL)
- Citric acid (1.46 mg/mL)
- Fumaric acid (0.13 mg/mL)
- Uric acid (0.40 mg/mL)
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HPLC Analytical Conditions: |
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|---|---|
| Column: | Develosil XG-C30M, 5 µm ( 4.6 x 150 mm) |
| Mobile Phase: | 50 mM Ammonium Phosphate (pH=2.0) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 210 nm |
| Injection Volume | 2.0 µL |
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