Analysis of 5-FU and Prodrugs

Sample:
- Gimeracil (0.55 mg/mL)
- 5-Fluouracil (0.54 mg/mL)
- Doxifluridine (0.58 mg/mL)
- Tegafur (0.55 mg/mL)
- Capecitabine (0.69 mg/mL)
- Camofur (0.66 mg/mL)
HPLC Analytical Conditions: |
|
---|---|
Column: | Develosil HSR C18, 5 µm ( 4.6 x 150 mm) |
Mobile Phase: | A) 25 mM (NH₄)₂HPO₄ (pH=7.0) B) Acetonitrile |
Gradient: | Further details here |
Flow Rate: | 1.0 mL/min |
Temperature: | 40°C |
Detection: | UV at 210 nm |
Injection Volume: | 2.0 µL |
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