Analysis of Pharmaceutical Ingredients (9 mixture) using UHPLC-UV

Analysis of Pharmaceutical Ingredients using UHPLC-UV

Sample:

  1. Pyridoxine HCl (0.10 mg/mL)
  2. I-phenylephrine (0.10 mg/mL)
  3. Acetaminophen (0.11 mg/mL)
  4. Guaifenesin (0.11 mg/mL)
  5. Diphenhydramine HCl (0.11 mg/mL)
  6. Ketoprofen (0.09 mg/mL)
  7. Flurbiprofen (0.10 mg/mL)
  8. Indomethacin (0.11 mg/mL)
  9. Ibuprofen (0.12 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR AQ C18, 2 µm ( 2.0 x 50 mm)
Mobile Phase:A) 0.1 % Phosphoric acid
B)  Acetonitrile + 0.1% Phosphoric acid
Gradient:Further details here
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 210 nm
Injection Volume:0.25 µL

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