Analysis of Sulfa Drugs using UHPLC-UV

Analysis of Sulfa Drugs using UHPLC-UV

Sample:

  1. Sulfadiazine (0.08 mg/mL)
  2. Sulfathiazole (0.10 mg/mL)
  3. Sulfapyridine (0.10 mg/mL)
  4. Sulfamerazine (0.09 mg/mL)
  5. Sulfamethazine (0.09 mg/mL)
  6. Sulfamethoxypyridazine (0.09 mg/mL)
  7. Sulfachloropyridazine (0.10 mg/mL)
  8. Sulfamethoxazole (0.10 mg/mL)
  9. Sulfadimethoxine (0.10 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR AQ C18, 2 µm ( 2.0 x 50 mm)
Mobile Phase:A) Water + 0.1% HCOOH
B) Acetonitrile + 0.1% HCOOH
Gradient:Further details here
Flow Rate:0.474 mL/min
Temperature:40°C
Detection:UV at 275 nm
Injection Volume:0.16 µL

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