Analysis of Sinigrin using HPLC-UV

Analysis of Sinigrin using HPLC-UV

Sample:

Sinigirin (0.1 mg/mL)

 

HPLC Analytical Conditions:
Column:  1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
2. Develosil HSR C1, 5 µm (4.6 x 150 mm)
3. Develosil C30-UG, 5 µm (4.6 x 150 mm)
4. Develosil ODS-HG, 5 µm (4.6 x 150 mm)
5. Develosil ODS, 5 µm (4.6 x 150 mm)
Mobile Phase: 10 mM Monopotassium Phosphate (pH=2.0)
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 210 nm
Injection Volume: 2.0 µL

 

 

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