Analysis of Food Additives in a Soft Drink using HPLC-UV

Analysis of Food Additives in Soft Drinks using HPLC-UV

Sample:

  1. Acesulfame K (0.50 mg/mL)
  2. Sodium Benzoate (0.49 mg/ml)
  3. Sodium Saccharin Dihydrate (0.54 mg/ml)
  4. Potassium Sorbate (0.45 mg/mL)
  5. Caffeine (0.46 mg/mL)
  6. Aspartame (0.46 mg/mL)
HPLC Analytical Conditions:
Column:  1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm) 2. Develosil HSR C18, 5 µm (4.6 x 150 mm) 3. Develosil HSR C1, 5 µm (4.6 x 150 mm)
Mobile Phase: A) 10 mM Diammonium Phosphate (pH 6.0) B)  Acetonitrile
Gradient: B) 10%-50% (0-10 min)
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 214 nm
Injection Volume: 1.0 µL

Download the product brochure or browse our technical library to find additional applications and publications.