Analysis of Water-Soluble Vitamins using HPLC-UV

Analysis of Water-Soluble Vitamins using HPLC-UV

Sample:

  1. Thiamin HCl (0.48 mg/mL)
  2. L(+)-Ascorbic acid (0.49 mg/mL)
  3. Nicotoinic Acid (0.49 mg/mL)
  4. Pyridoxine HCl (0.46 mg/mL)
  5. Nicotinamide (0.50 mg/mL)
  6. Calcium (+)-pantothenate (0.65 mg/mL)
  7. Cyanocobalamin (0.46 mg/mL)
  8. Folic acid (0.46 mg/mL)
  9. Riboflavin sodium phosphate (0.46 mg/mL)
  10. (+)-Biotin (0.47 mg/mL)

HPLC Analytical Conditions:

Column:  Develosil HSR AQ C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase: A) 25mM Ammonium phosphate monobasic
B) Methanol
Gradient: Further details here
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 210 nm
Injection Volume: 10 µL

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