Analysis of Flavonoids using HPLC-UV

Analysis of Flavonoids using HPLC-UV

Sample:

  1. Puearin (0.025 mg/mL)
  2. Daidzein (0.025 mg/mL)
  3. Biochanin A (0.027 mg/mL)
  4. Ipriflavone (0.025 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR AQ C18, 3 µm ( 2.0 x 50 mm)
Mobile Phase:A) 0.2% HCOOH in Water
B) 0.2% HCOOH in Acetonitrike
Gradient:Further details here
Flow Rate:0.35 mL/min
Temperature:40°C
Detection:UV at 254 nm
Injection Volume:1.0 µL

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