Analysis of Peptide using HPLC

Analysis of Peptide using HPLC

Sample:

  1. Gly-Tyr (0.05 mg/mL)
  2. Val-Tyr-Val (0.05 mg/mL)
  3. Met-Enkephalin (0.05 mg/mL)
  4. Angiotensin II (0.05 mg/mL)
  5. Leu-Enkephalin (0.05 mg/mL)

HPLC Analytical Conditions:

Column: 1. Develosil HSR C1, 5 µm (4.6 x150 mm)
2. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
Mobile Phase:A) 25 mM NaCl (pH=3.0)
B) Acetonitrile
Gradient:Further details here
Flow Rate:1.0 mL/min
Temperature:40°C
Mixer Volume:10 µL

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