Analysis of Purine Derivatives using HPLC-UV

Analysis of Purine Derivatives using HPLC-UV Figure 1
Analysis of Purine Derivatives using HPLC-UV Figure 2

Sample:

  1. Allantoin (0.42 mg/mL)
  2. Hypoxanthine (0.11 mg/mL)
  3. Uric acid (0.12 mg/mL)
  4. Xanthine (0.11 mg/mL)
  5. Inosine (0.11 mg/mL)

HPLC Analytical Conditions:

Column: 1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
2. Develosil HSR C1, 5 µm (4.6 x 150 mm)
Mobile Phase:A) 25 mM NH₄H₂PO₄ (pH=2.0)
B) Acetonitrile
Gradient:Further details here
Flow Rate:1.0 mL/min
Temperature:30°C
Detection:UV at 210 nm
Injection Volume:1.0 µL

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