Analysis of Nucleic Acid Related Compounds using HPLC-UV

Analysis of Nucleic Acid Related Compounds using HPLC-UV

Sample:

  1. Cytosine (0.05 mg/mL)
  2. Uracil (0.05 mg/mL)
  3. Cytidine (0.05 mg/mL)
  4. Guanine (0.08 mg/mL)
  5. Uridine (0.06 mg/mL)
  6. Thymine (0.05 mg/mL)
  7. Adenine (0.06 mg/mL)
  8. Guanosine (0.06 mg/mL)
  9. Thymidine (0.06 mg/mL)
  10. Adenosine (0.05 mg/mL)

HPLC Analytical Conditions:

Column: 1. Develosil HSR AQ C18, 5 µm (4.6x150 mm)
2. Develosil HSR AQ C18, 3 µm (4.6x75 mm)
Mobile Phase:A) 50 mM HCOONH₄ (pH=7.0)
B) Acetonitrile
Gradient:Further details here
Flow Rate:1.0 mL/min
Temperature:30°C
Detection:UV at 254 nm
Injection Volume:2.0 µL

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