Analysis of 5-FU and Prodrugs

Analysis of 5-FU and Prodrugs using HPLC-UV

Sample:

  1. Gimeracil (0.55 mg/mL)
  2. 5-Fluouracil (0.54 mg/mL)
  3. Doxifluridine (0.58 mg/mL)
  4. Tegafur (0.55 mg/mL)
  5. Capecitabine (0.69 mg/mL)
  6. Camofur (0.66 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase:A) 25 mM (NH₄)₂HPO₄ (pH=7.0)
B) Aceonitrile
Gradient:Further details here
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 210 nm
Injection Volume:2.0 µL

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