Analysis of Sulfa Drugs using HPLC-UV

Analysis of Sulfa Drugs using HPLC-UV

Sample:

  1. Sulfadiazine (0.04 mg/mL)
  2. Sulfathiazole (0.05 mg/mL)
  3. Sulfapyridine (0.06 mg/mL)
  4. Sulfamerazine (0.06 mg/mL)
  5. Sulfamethazine (0.06 mg/mL)
  6. Sulfamethoxypyridazine (0.06 mg/mL)
  7. Sulfachloropyridazine (0.05 mg/mL)
  8. Sulfamethoxazole (0.05 mg/mL)
  9. Sulfamethoxine (0.08 mg/mL)

HPLC Analytical Conditions:

Column: 1. Develosil HSR C18, 5 µm ( 4.6 x 150 mm)
2. Develosil HSR C18, 3 µm (4.6 x 100 mm)
Mobile Phase:A) Water + 0.1% HCOOH
B)  Methanol + 0.1% HCOOH
Gradient:Further details here
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 275 nm
Injection Volume:2.0 µL

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